中国糖料2026，Vol.48Issue(1)：40-46,7.DOI:10.13570/j.cnki.scc.2026.01.005
基于Touchdown程序的甜菜SRAP核心引物的筛选
Screening of SRAP Core Primers for Sugar Beet Based on Touchdown Program
摘要
Abstract
[Objective]The main objective is to select SRAP core primers with high polymorphism and high stability for the identification of genetic diversity in sugar beet.[Methods]Amplification was performed using SRAP primer combinations under a Touchdown program.The products were separated by 8%non-denaturing polyacrylamide gel electrophoresis,and primers meeting the criteria were selected based on data analysis of the amplification results.[Results]From 504 pairs consisting of 24 upstream primers and 21 downstream primers,43 pairs of primer combinations with high polymorphism and stability were identified,of which 40 pairs of primer combinations had a polymorphism information content(PIC)value greater than 0.75,24 pairs of primer combinations had a PIC value greater than 0.8,and 4 pairs of primer combinations had a PIC value greater than 0.9.The PIC range was 0.657 to 0.953,and the average PIC value of primer pairs was 0.812.[Conclusion]The screening of SRAP core primers provides theoretical support for the identification of genetic diversity in sugar beet,as well as for the selection of parents for further cross-breeding and molecular marker-assisted breeding.关键词
甜菜/SRAP引物/分子标记/核心引物Key words
sugar beet/SRAP primers/molecular markers/core primers分类
农业科技引用本文复制引用
张程伟,刘佳俊,阚文亮,宋玥,吴则东..基于Touchdown程序的甜菜SRAP核心引物的筛选[J].中国糖料,2026,48(1):40-46,7.基金项目
大学生创新创业训练计划项目"适宜于甜菜遗传多样性鉴定的SRAP核心引物的筛选及其应用"(S202410212150) （S202410212150）
国家现代农业产业技术体系(糖料)项目(CARS-17)资助. （糖料）
