中国现代医学杂志2026，Vol.36Issue(2)：23-30,8.DOI:10.3969/j.issn.1005-8982.2026.02.004
前列腺素E2通过EP4受体调控甲状腺乳头状癌细胞增殖与侵袭的机制研究
Mechanism study of prostaglandin E2 regulating proliferation and invasion of papillary thyroid carcinoma cells via the EP4 receptor
摘要
Abstract
Objective To investigate the role of prostaglandin E2(PGE2)in the pathogenesis of papillary thyroid carcinoma and its underlying mechanisms.Methods Human thyroid follicular cells(Nthy-ori 3-1)and papillary thyroid carcinoma cells(TPC-1)were cultured.The concentration of PGE2 in the cell culture supernatant was measured by ELISA.The expression of prostaglandin E2 receptor 4(EP4)in both cell lines was detected by Western Blotting and qRT-PCR.TPC-1 cells were treated with 0 μmol/L,1 μmol/L,2 μmol/L,or 5 μmol/L PGE2,cell viability was assessed using the CCK-8 assay,and EP4 protein expression was detected by Western Blotting.TPC-1 cells were treated with 0 μmol/L or 5 μmol/L PGE2,and cell proliferation and invasion capabilities were evaluated using the CCK-8 assay and Transwell assay,respectively.Furthermore,TPC-1 cells were treated with 0 μmol/L PGE2,5 μmol/L PGE2,5 μmol/L PGE2 combined with 5 μmol/L EP4 receptor agonist(receptor agonist 2),or 5 μmol/L PGE2 combined with 5 μmol/L EP4 receptor inhibitor(L-161982),and cell proliferation and invasion capabilities were subsequently measured using the CCK-8 assay and Transwell assay.Results The PGE2 content in the culture supernatant of TPC-1 cells was higher than that of Nthy-ori 3-1 cells(P<0.05).The mRNA expression level of the EP4 receptor in TPC-1 cells was higher than that in Nthy-ori 3-1 cells(P<0.05).The protein expression level of the EP4 receptor in TPC-1 cells was higher than that in Nthy-ori 3-1 cells(P<0.05).The cell viability in the 5 μmol/L PGE2 group was higher than that in the 0 μmol/L PGE2 group(P<0.05).A repeated-measures ANOVA was conducted to compare the relative proliferation rates of cells in the 0 μmol/L and 5 μmol/L PGE2 groups at 24,48,72,and 96 h,and the results showed that the differences in relative proliferation rates among different time points were statistically significant(P<0.05)and that the differences in relative proliferation rates between the two groups were statistically significant(P<0.05).The differences in the change trends of relative proliferation rates between the two groups were also statistically significant(P<0.05).The number of cells passing through the membrane in the 5 μmol/L PGE2 group at 24 h was higher than that in the 0 μmol/L PGE2 group(P<0.05).The relative expression levels of EP4 protein in the 2 μmol/L and 5 μmol/L PGE2 groups were higher than those in the 0 μmol/L PGE2 group(P<0.05).A repeated-measures ANOVA was performed to compare the relative proliferation rates of cells in the 0 μmol/L PGE2 group,5 μmol/L PGE2 group,5 μmol/L PGE2+5 μmol/L EP4 receptor agonist 2 group,and 5 μmol/L PGE2+5 μmol/L EP4 L-161982 group at 24,48,72,and 96 h,and the results showed that the differences in relative proliferation rates among different time points were statistically significant(P<0.05)and that the differences in relative proliferation rates among the groups were statistically significant(P<0.05).The differences in the change trends of relative proliferation rates among the groups were also statistically significant(P<0.05).The number of cells passing through the membrane in the 5 μmol/L PGE2+EP4 receptor agonist 2 group was higher than that in the 5 μmol/L PGE2 group(P<0.05),while the number of cells passing through the membrane in the 5 μmol/L PGE2+EP4 L-161982 group was lower than that in the 5 μmol/L PGE2 group(P<0.05).Conclusion PGE2 promotes proliferation and invasion of TPC-1 cells via the EP4 receptor.关键词
甲状腺乳头状癌/前列腺素E2/EP4受体Key words
thyroid cancer/prostaglandin E2/EP4 receptor分类
医药卫生引用本文复制引用
田颖,刘文静,许爱梅,王芳,张方华..前列腺素E2通过EP4受体调控甲状腺乳头状癌细胞增殖与侵袭的机制研究[J].中国现代医学杂志,2026,36(2):23-30,8.基金项目
国家自然科学基金面上项目(No:82270829) （No:82270829）
