中国现代医学杂志2026，Vol.36Issue(2)：55-60,6.DOI:10.3969/j.issn.1005-8982.2026.02.009
特应性皮炎患儿与健康儿童肠道菌群、炎症因子水平的差异分析
Differences in gut microbiota and inflammatory factor levels between children with atopic dermatitis and healthy controls
摘要
Abstract
Objective To investigate the differences in gut microbiota and inflammatory factor levels between children with atopic dermatitis and healthy controls.Methods A total of 116 children with atopic dermatitis who received treatment in the First Affiliated Hospital of Ningbo University from January 2022 to January 2024 were selected as the observation group,and 102 healthy children who underwent health checkups at the hospital during the same period were enrolled as the control group.Fecal samples from both groups were collected,and 16S rRNA sequencing and Qiime software were used to analyze the α-diversity of gut microbiota,including Chao1 index,number of observed species(Sobs),Shannon index,and Simpson index.Enzyme-linked immunosorbent assay(ELISA)was employed to detect the levels of inflammatory factors,including interleukin-4(IL-4),IL-13,and IL-31.Pearson correlation analysis was performed to explore the relationship between gut microbiota diversity and inflammatory factor levels,and receiver operating characteristic(ROC)curves were applied to evaluate the diagnostic value of gut microbiota α-diversity and inflammatory factor levels for children with atopic dermatitis,respectively.Results The Chao1,Sobs,Shannon,and Simpson indices in the observation group were significantly lower than those in the control group(P<0.05),while the levels of IL-4,IL-13 and IL-31 were significantly higher in the observation group(P<0.05).Pearson correlation analysis revealed negative correlations between gut microbiota α-diversity indices and serum inflammatory factor levels(P<0.05).ROC curve analysis indicated that the combination of gut microbiota α-diversity and inflammatory factors for diagnosing atopic dermatitis yielded a sensitivity of 98.3%(95%CI:0.939,0.998),with a specificity of 98.0%(95%CI:0.931,0.998)and an area under the curve of 0.973(95%CI:0.946,1.000),outperforming each individual marker.Conclusion Children with atopic dermatitis exhibit reduced gut microbiota α-diversity and elevated inflammatory factor levels,and combined detection of these indicators can enhance the diagnostic efficacy for atopic dermatitis.关键词
特应性皮炎/肠道菌群/炎症因子/相关性分析/诊断效能Key words
atopic dermatitis/gut microbiota/inflammatory factors/correlation analysis/diagnostic efficacy分类
医药卫生引用本文复制引用
胡雨嫣,李志飞..特应性皮炎患儿与健康儿童肠道菌群、炎症因子水平的差异分析[J].中国现代医学杂志,2026,36(2):55-60,6.基金项目
浙江省医药卫生科技计划项目(No:2023KY1108) （No:2023KY1108）
浙江省卫生健康科技计划项目(No:2021KY1044) （No:2021KY1044）
