中国畜牧兽医2026，Vol.53Issue(1)：1-14,14.DOI:10.16431/j.cnki.1671-7236.2026.01.001
基因编辑技术及其在猪育种中的应用研究进展
Research Progress on Gene Editing Technology and Its Application in Breeding of Pigs
摘要
Abstract
Gene editing is an important tool for precise modification of specific gene sequences in the genome to achieve molecular breeding.The rapid development of gene editing technology has provided a new approach for modern molecular breeding in pigs,significantly improving breeding efficiency.This technology has undergone three generations of evolution:①Zinc finger nuclease(ZFNs)and transcription activator-like effector nucleases(TALENs)laid the foundation for targeted editing but had the drawbacks of complex operation and high cost.②The clustered regularly interspaced short palindromic repeats(CRISPR)system made a breakthrough,among which iGeoCas9 improved thermal stability and editing activity by a hundredfold through structural modification,and the miniaturization of Cas protein optimized delivery efficiency.③Base editors achieved precise single-base conversion,and the guide editing system broke through type limitations,supporting free base conversion and small fragment modification.In pig breeding,this technology improves meat quality,enhances growth performance,optimizes reproductive efficiency,and strengthens disease resistance,it also enables innovative applications such as cold resistance and environmental protection.Compared with traditional breeding,gene editing technology can shorten the cycle and maintain genetic diversity,but it faces challenges such as insufficient editing efficiency,off-target risks,and industrialization barriers.This article systematically reviews the gene editing technological development and breeding applications,aiming to provide references for optimizing the performance of editing tools and establishing a safe industrialization system,promoting technological innovation,and supporting the progress of pig breeding technology and the sustainable development of the industry.关键词
基因编辑/CRISPR/猪/育种Key words
gene editing/CRISPR/pigs/breeding分类
农业科技引用本文复制引用
张寒冰,郭亚苹,张家庆,任巧玲,陈俊峰,刘付玖,王璟,邢宝松..基因编辑技术及其在猪育种中的应用研究进展[J].中国畜牧兽医,2026,53(1):1-14,14.基金项目
国家重点研发计划项目(2021YFD1301200) （2021YFD1301200）
河南省重点研发专项(251111110200) （251111110200）
中央引导地方科技发展资金(Z20241471127) （Z20241471127）
河南省农业科学院自主创新项目(2025ZC63) （2025ZC63）
河南省农业科学院科技创新团队项目(2025TD37) （2025TD37）
