中国畜牧兽医2026，Vol.53Issue(1)：190-200,11.DOI:10.16431/j.cnki.1671-7236.2026.01.017
大口黑鲈稚鱼对维生素B6需求量的研究
Study on Vitamin B6 Requirements of Juvenile Largemouth Bass(Micropterus salmoides)
摘要
Abstract
[Objective]This study aimed to investigate the effects of dietary vitamin B6(VB6)contents on the growth performance,biochemical parameters,and digestive and antioxidant capacity in juvenile largemouth bass(Micropterus salmoides),and determine the optimal dietary VB6 requirement,providing nutrient requirements parameters and theoretical basis for the development of formulated feed in juvenile largemouth bass.[Method]A total of 2 400 juvenile largemouth bass with an initial body weight of(92.50±0.28)mg were randomly divided into six groups:P0(control group),P1,P2,P3,P4,and P5,with four replicates per group and 100 fish per replicate,which were fed six types of formulated feed containing 3.66,9.89,17.80,31.03,46.36,and 58.91 mg/kg VB6,respectively,for a 30 days culture period.After the test,the effects of VB6 on the growth performance,biochemical parameters,and digestive and antioxidant capacity in juvenile largemouth bass were measured,and the water stress test was carried out.[Result]The weight gain and specific growth rates of juvenile largemouth bass in P1 group were significantly higher than those in P4 and P5 groups(P<0.05),and there was no significant difference in feed coefficient(P>0.05).The crude protein content of juvenile largemouth bass in P4 group was significantly higher than that in P2 group(P<0.05),the crude fat content was significantly lower than that in P0,P1,and P3 groups(P<0.05).There was no significant difference in the contents of glucose and triglycerides,and alanine aminotransferase activity in visceral mass of juvenile largemouth bass in each group(P>0.05).The aspartate aminotransferase activity in P4 and P5 groups was significantly higher than that in P0 group(P<0.05).The intestinal muscular thickness of juvenile largemouth bass in P2,P3,and P5 groups was significantly lower than that in P0 group(P<0.05).The activities of trypsin and α-amylase in P4 and P5 groups were significantly lower than those in P0 group(P<0.05),while the lipase activity in P1 group was significantly higher than that in other groups(P<0.05).The survival rate of juvenile largemouth bass in P2-P5 groups was significantly lower than that in P1 group post-air-exposure stress(P<0.05),the total superoxide dismutase(T-SOD)activity in visceral mass was significantly lower than that in P0 and P1 groups(P<0.05),and the catalase(CAT)activity was significantly lower than that in P0 group(P<0.05).The malondialdehyde(MDA)content in P3 group was significantly lower than that in P0 group(P<0.05).Compared with pre-air-exposure stress,the T-SOD activity and MDA content in visceral mass of juvenile largemouth bass in each group were significantly increased post-air-exposure stress(P<0.05),while CAT activity only significantly increased in P0-P2 groups(P<0.05).[Conclusion]Under the conditions of this experiment,line regression analysis showed that the VB6 requirement of juvenile largemouth bass in feed was 5.34 mg/kg using weight gain rate as the evaluation index,and the optimal amount of VB6 addition to the feed was 9.89 mg/kg with survival rate after post-air exposure as the evaluation index.关键词
大口黑鲈稚鱼/维生素B6/生长性能/生化指标/抗氧化能力Key words
juvenile largemouth bass/VB6/growth performance/biochemical indicators/antioxidant capacity分类
农业科技引用本文复制引用
梁浩辉,覃瑶,熊攀,曹俊明,蔡佳,胡俊茹,陈晓瑛,王国霞..大口黑鲈稚鱼对维生素B6需求量的研究[J].中国畜牧兽医,2026,53(1):190-200,11.基金项目
广州市基础研究计划民生科技专题项目(202002020018) （202002020018）
