中国药房2026，Vol.37Issue(1)：24-29,6.DOI:10.6039/j.issn.1001-0408.2026.01.05
黑果枸杞改善睡眠的作用及机制研究
Study on the effects and mechanisms of Lycium ruthenicum Murr.in improving sleep
摘要
Abstract
OBJECTIVE To investigate the effects and mechanisms of Lycium ruthenicum Murr.in improving sleep.METHODS Network pharmacology was employed to identify the active components of L.ruthenicum and their associated disease targets,followed by enrichment analysis.A caffeine-induced zebrafish model of sleep deprivation was established,and the zebrafish were treated with L.ruthenicum Murr.extract(LRME)at concentrations of 0.1,0.2 and 0.4 mg/mL,respectively;24 h later,behavioral changes of zebrafish and pathological alterations in brain neurons were subsequently observed.The levels of inflammatory factors[interleukin-6(IL-6),IL-1β,IL-10,tumor necrosis factor-α(TNF-α)],oxidative stress markers[superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),catalase(CAT)],and neurotransmitters[5-hydroxytryptamine(5-HT),γ-aminobutyric acid(GABA),glutamic acid(Glu),dopamine(DA),and norepinephrine(NE)]were measured.The protein expression levels of protein kinase B1(AKT1),phosphorylated AKT1(p-AKT1),epidermal growth factor receptor(EGFR),B-cell lymphoma 2(Bcl-2),sarcoma proto-oncogene,non-receptor tyrosine kinase(SRC),and heat shock protein 90α family class A member 1(HSP90AA1)in the zebrafish were also determined.RESULTS A total of 12 active components and 176 intersecting disease targets were identified through network pharmacology analysis.Among these,apigenin,naringenin and others were recognized as core active compounds,while AKT1,EGFR and others served as key targets;EGFR tyrosine kinase inhibitor resistance signaling pathway was identified as the critical pathway.The sleep improvement rates in zebrafish of LRME low-,medium-,and high-dose groups were 54.60%,69.03%and 77.97%,respectively,while the inhibition ratios of locomotor distance were 0.57,0.83 and 0.95,respectively.Compared with the model group,the number of resting counts,resting time and resting distance were significantly increased/extended in LRME medium-and high-dose groups(P＜0.05).Neuronal damage in the brain was alleviated.Additionally,the levels of IL-6,IL-1β,TNF-α,MDA,Glu,DA and NE,as well as the protein expression levels of AKT1,p-AKT1,EGFR,SRC and HSP90AA1,were markedly reduced(P＜0.05),while the levels of IL-10,SOD,GSH-Px,CAT,5-HT and GABA,as well as Bcl-2 protein expression,were significantly elevated(P＜0.05).CONCLUSIONS L.ruthenicum Murr.demonstrates sleep-improving effects,and its specific mechanism may be related to the regulation of inflammatory responses,oxidative stress,neurotransmitter balance,and the EGFR tyrosine kinase inhibitor resistance signaling pathway.关键词
黑果枸杞/改善睡眠/失眠/网络药理学/斑马鱼Key words
Lycium ruthenicum Murr./improving sleep/insomnia/network pharmacology/zebrafish分类
医药卫生引用本文复制引用
谯明,杨建华,胡君萍,赵耀,朱毅,曹叶霞,文丽梅,巩月红,李想,汪娟成,王涛..黑果枸杞改善睡眠的作用及机制研究[J].中国药房,2026,37(1):24-29,6.基金项目
新疆维吾尔自治区科技创新领军人才项目(No.2024TSYCLJ0048) （No.2024TSYCLJ0048）
新疆维吾尔自治区自然科学基金重点项目(No.2022D01D14) （No.2022D01D14）
