中国药理学通报2026，Vol.42Issue(1)：164-171,8.DOI:10.12360/CPB202507021
漆黄素靶向EGFR/SRC/Bax改善抑郁样损伤的作用及机制
Effect of fisetin targeting EGFR/SRC/Bax on improving depressive-like damage and its mechanism
摘要
Abstract
Aim To explore the potential targets and molecular biological mechanisms underlying the anti-depressant effects of fisetin.Methods Targets re-lated to fisetin and depression were screened.CCK-8 assay was used to determine the optimal concentration of corticosterone for inducing a depression model in PC12 cells and the appropriate administration concen-tration of fisetin.Western blot analysis was employed to assess the expression levels of epidermal growth fac-tor receptor(EGFR),SRC,and Bax proteins in PC12 cells.Immunofluorescence experiments were con-ducted to observe the localization and expression of EGFR and SRC proteins.Forced swimming test,tail suspension test,and elevated plus maze test were used to evaluate depressive-like behaviors in mice,while HE staining was utilized to observe morphological changes in mouse brain tissue.Results Corticoste-rone significantly upregulated the expression of EGFR,SRC,and Bax proteins in PC12 cells,whereas fisetin intervention notably downregulated the expression levels of these proteins.Compared with the control group,mice in the chronic restraint stress(CRS)model group exhibited pronounced depressive-like behaviors,while these depressive-like impair-ments were ameliorated in the fluoxetine group and the high-and low-dose fisetin groups.Conclusion Fise-tin may reduce neuronal damage and ultimately im-prove depressive-like behaviors in mice by modulating the expression of EGFR/SRC/Bax proteins.关键词
漆黄素/抑郁症/EGFR/SRC/网络药理学/分子动力学模拟Key words
fisetin/depression/EGFR/SRC/net-work pharmacology/molecular dynamics simulation分类
医药卫生引用本文复制引用
马昊,武涵涵,杨锦霞,冯袁辉,马槊乾,赵喜庆,商学良,秦丽娟..漆黄素靶向EGFR/SRC/Bax改善抑郁样损伤的作用及机制[J].中国药理学通报,2026,42(1):164-171,8.基金项目
唐山市科技计划项目(No 25130221B) （No 25130221B）
河北省中医药管理局科学研究项目(No 2026102,2022553) （No 2026102,2022553）
河北省医学科学研究项目(No 20260654,20221514) （No 20260654,20221514）
国家自然科学基金资助项目(No 32500476) （No 32500476）
