中国油料作物学报2025，Vol.47Issue(6)：1387-1398,12.DOI:10.19802/j.issn.1007-9084.2024302
甘蓝型油菜耐盐性全基因组关联分析与种质筛选
Genome wide association study and germplasm screening of salt tolerance in Brassica napus L
摘要
Abstract
Brassica napus(L.)is an dominant crop for improving and utilizing saline alkali land.To explore the salt tolerant functional genes and germplasm of rapeseed,in this study,we treated 350 rapeseed accession popula-tions with salt stress during the germination stages,and recorded the germination force,germination rate,root length ratio,hypocotyl length ratio,and fresh weight ratio under salt stress.Genome wide association study was performed by combining SNP markers based on whole genome resequencing.A total of 128 significantly associated SNPs were obtained and located in 43 candidate intervals.Among them,6 candidate intervals contain more than 5 SNPs,dis-tributed on chromosomes A01,A02,A09,and C08,which are the key candidate intervals in this study.Subsequent-ly,combined with salt stress transcriptome data and gene annotation,preliminary analysis was conducted on the key genes involved in the key candidate intervals.Based on various salt tolerance indicators,5 salt tolerant and 5 salt sensitive germplasm resources were screened.In conclusion,this study established a complete salt tolerance evalua-tion system for rapeseed germination stages.Through GW AS analysis,candidate regions closely related to salt toler-ance in rapeseed were identified,and rapeseed germplasm resources with extreme salt tolerance and sensitivity were screened.This study provides a reference for the exploration of salt tolerance functional genes and germplasm identi-fication in rapeseed.关键词
油菜/耐盐性/GWAS/种质资源Key words
Brassica napus L./salt tolerance/GWAS/germplasm resource分类
农业科技引用本文复制引用
黄紫红,范世航,华玮,付正伟..甘蓝型油菜耐盐性全基因组关联分析与种质筛选[J].中国油料作物学报,2025,47(6):1387-1398,12.基金项目
农业生物育种国家科技重大专项(2022ZD04010) （2022ZD04010）
湖北省自然科学基金(2023AFB358) （2023AFB358）
武汉市知识创新专项(2023020201020396) （2023020201020396）
中央级科研院所基本科研业务费专项(1610172023004) （1610172023004）
