中国油料作物学报2025，Vol.47Issue(6)：1399-1407,9.DOI:10.19802/j.issn.1007-9084.2024232
皖豆15抗裂荚突变体的综合利用
Comprehensive utilization of soybean pod shattering resistance mutant originated from Wandou 15
摘要
Abstract
To improve the pod shattering resistance of soybean varieties and create research materials,60Co-γ-ray and EMS mutagen were successively used on Wandou 15 which is widely cultivating in production but suffering from severe pod shattering phenotype.Then we conducted a series of experiments such as single seed propagation,pod shattering resistance screening,observation of pod belly suture slices during development,investigation of mu-tant agronomic traits,identification of mutant fingerprint maps,and genotype analysis of known pod shattering genes.Research found that the mutant MT23045 has a good pod shattering resistance phenotype.There is little difference in other agronomic traits except for a slight increase in plant height and node number and the yield has slightly in-creased compared to the control.The thickness of the seam on the belly of the pods 27 days after flowering increased by 143％.Compared with the wild-type material,there are 5 DNA markers differences(3 markers fall in the soybean pod shattering linkage group).Finally there were no mutations reported in the two pod shattering gene regions.Our research findings provide usable germplasm resources for soybean pod shattering resistance breeding,and MT23045 is also an ideal research material for discovering new pod shattering resistance genes in soybean.关键词
大豆/抗裂荚/突变体/分子标记Key words
soybean/pod shattering resistance/mutant/molecular markers分类
农业科技引用本文复制引用
余希文,李曙光,王亚琪,傅蒙蒙,赵志鑫,徐海风..皖豆15抗裂荚突变体的综合利用[J].中国油料作物学报,2025,47(6):1399-1407,9.基金项目
江苏省揭榜挂帅项目优质抗病鲜食大豆新品种选育及应用(JBGS[2021]057) （JBGS[2021]057）
钟山实验室生物育种项目(ZSBBL-KY2023-03) （ZSBBL-KY2023-03）
淮安市自然科学项目联合专项(HABL202226) （HABL202226）
国家自然科学基金青年基金(32201729) （32201729）
淮安市农业科学研究院科研发展基金(HNY202112) （HNY202112）
