中国油料作物学报2025，Vol.47Issue(6)：1408-1419,12.DOI:10.19802/j.issn.1007-9084.2024204
江西芝麻核心种质的表型及分子多样性分析
Phenotypic and molecular diversity analysis of core sesame germplasm in Jiangxi Province
摘要
Abstract
To efficiently utilize sesame germplasm,we analyzed genetic diversity among core sesame germ-plasm of Jiangxi.In the study,we used 110 sesame core germplasm(constructed from 736 sesame germplasm re-sources in 78 counties of Jiangxi Province)as materials,to perform diversity and evaluation on their 25 phenotypic traits.Genetic diversity index,coefficient of variation,correlation,principal component analysis,and clustering,and molecular detection were studied by using 25 pairs of polymorphic SSR primers.Results showed that genetic diversi-ty index of the 25 traits was 0.5573 to 2.0687,variation coefficient was 4.83％to 48.16％,basically close to that of the original collection population.The variation coefficients of 7 traits of 12 quantitative traits were more than 20％.Cluster analysis divided the tested germplasm into 4 categories.Among them,Group Ⅰ could be used as a potential parent material for breeding new varieties with dwarf and high-yield,Group Ⅱ as a whole belongs to potential low-yield germplasm resources,which should be avoided in the breeding process,Group Ⅲ could be used as the poten-tial parent material for breeding new varieties with multiple capsules and high yield,Group Ⅳ could be used as po-tential parent for breeding varieties with multiple capsules.106 DNA bands were amplified from the core germ-plasm with 25 SSR primer pairs,resulting polymorphic ratio of 100％.The polymorphic bands amplified by each primer pair averaged 4.36.The variation range of primer polymorphism information content value was 0.0476-0.3606,with an average of 0.2609.The cluster analysis showed the variation range was 0.4528-0.9717,with an av-erage of 0.6900,and the genetic distance was 0.0283-0.5472,with an average of 0.3100.Given all the core germ-plasm be divided into 3 regions by different geographical directions,the overall genetic similarity was as follows:Northern Jiangxi＞Southern Jiangxi＞Central Jiangxi.The genetic diversity in central Jiangxi was more abundant than that in northern and southern regions,which might be associated with the frequent circulation of germplasm in central Jiangxi to both northern and southern locations.According to seed color,it was divided into white sesame,black sesame and other seed color sesame.From comparison of genetic similarity coefficient and genetic distance,it could be concluded that the genetic diversity of other seed color sesame germplasm is abundant,followed by white sesame.The genetic diversity of black sesame germplasm was relatively poor.关键词
江西省/芝麻/核心种质/表型性状/分子标记/多样性分析Key words
Jiangxi/seasame/core germplasm/phenotypic/molecular marker/diversity analysis分类
农业科技引用本文复制引用
王郅琪,颜小文,梁俊超,曾攀,乐美旺,周红英,孙建..江西芝麻核心种质的表型及分子多样性分析[J].中国油料作物学报,2025,47(6):1408-1419,12.基金项目
国家现代农业产业技术体系(CARS-14) （CARS-14）
江西省农作物良种联合攻关项目 （）
江西省重点研发计划项目(20212BBF63011) （20212BBF63011）
