中国油料作物学报2025，Vol.47Issue(6)：1420-1430,11.DOI:10.19802/j.issn.1007-9084.2024122
油莎豆茎豆油脂积累的规律分析与关键基因挖掘
Regularity analysis and key gene mining of oil accumulation in Cyperus esculentus tubers
摘要
Abstract
In order to clarify regularity of oil accumulation in Cyperus esculentus tubers and to mine key genes related,high-oil cultivar Yuyousha 2 was used as material.Tubers were labeled with a double-layer(inner layer transparent)sand culture device,and then changes of oil content in tubers at different developmental stages were an-alyzed.Expression changes of core lipid anabolic pathway related genes and all transcription factor genes were ex-plored through transcriptome sequencing of tubers at various developmental stages.Results showed that oil accumu-lation in C.esculentus tubers mainly lasted from 10 d to 50 d of tuber development,presenting an'S'accumulation pattern.Moreover,oil accumulating rate gradually increased before 30 d of tuber development,and then gradually decreased.Through transcriptome sequencing of tubers at 10,20,30,40 and 50 days of tuber development,it was found that main genes related to plant core lipid anabolic pathway are expressed in different degrees in C.esculen-tus.Gene expression changes in tubers at multiple stages showed that,30 core genes related to core lipid anabolism pathway,and 23 transcription factor genes were screened out for their consistency with oil accumulation trend,in-cluding key functional genes with extremely high expression levels such as Oleosin,Caleosin,FAD,ACP,PDHC,ACCase and SAD,as well as AP2 family transcription factor gene WRI1 involved in oil accumulation,which indicat-ing that these genes are might to be involved in oil accumulation regulation in Cyperus esculentus.关键词
油莎豆/油脂积累/关键基因/转录因子Key words
Cyperus esculentus/oil accumulation/key gene/transcription factor分类
农业科技引用本文复制引用
张向歌,王会伟,陈晨,朱雅婧,程珊,李春鑫,宋万献,翟允汝,宋雯栩,李居政..油莎豆茎豆油脂积累的规律分析与关键基因挖掘[J].中国油料作物学报,2025,47(6):1420-1430,11.基金项目
河南省重大科技专项(211100110100) （211100110100）
河南省重点研发与推广专项(科技攻关)项目(242102110305) （科技攻关）
河南省农业科学院新兴学科发展专项(2024XK02) （2024XK02）
