摘要
Abstract
Objective:To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of asperosaponin Ⅵ and tanshinone ⅡA in Yigong Granules,in order to improve the quality control standard of this preparation.Methods:HPLC analysis was performed using an Agilent Eclipse Plus C18 column(4.6 mum ×250 mm,5 μm).The mobile phase con-sisted of 0.1％(v/v)phosphoric acid solution(A)and acetonitrile(B),with gradient elution according to a specified program.The flow rate was set at 1.0 mL·min-1,and the column temperature was maintained at 30 ℃.Detection wavelengths were set at 210 nm for asperosaponin Ⅵ and 270 nm for tanshinone ⅡA,based on their ultraviolet absorption characteristics.The injection volume was 10 μL.Results:Asperosaponin Ⅵ showed a good linear relationship within the concentration range of 23.575 0-707.250 0 μg·mL-1,with a correlation coefficient(r2)of 1.000 0.The average recovery rate was 96.4％,and the relative standard deviation(RSD)was 1.8％(n=6).Tanshinone ⅡA also exhibited a good linear relationship within the concentration range of 1.483 5-44.505 0 μg·mL-1,with an r2 of 1.000 0.The average recovery rate was 94.8％,and the RSD was 1.5％(n=6).Conclusion:A simple,accurate,reproduci-ble,and stable HPLC method was successfully developed for the simultaneous and rapid determination of asperosaponin Ⅵ and tanshinoneⅡA in Yigong Granules.This method is reliable and practical,making it suitable for multi-component quality evaluation of the preparation.关键词
益宫颗粒/高效液相色谱/川续断皂苷Ⅵ/丹参酮ⅡA/质量控制Key words
Yigong granules/High performance liquid chromatography(HPLC)/Asperosaponin Ⅵ/Tanshinone ⅡA/Quality control分类
医药卫生
