中国药物评价2025，Vol.42Issue(6)：436-440,5.
基于标准加入法对胖大海中黄曲霉毒素B1含量测定的研究
Research on the Determination of Aflatoxin B1 Content in Sterculiae Lychnophorae Semen by Standard Addition Method
摘要
Abstract
Objective:The content of aflatoxin B,in Sterculiae Lychnophorae Semen was determined by the standard addition method,and the advantages and limitations of this method were analyzed.Methods:Using high-performance liquid chromatography with post-col-umn photochemical derivatization(HPLC-FLD)as the detection method,the standard addition method was analyzed the content of aflatox-in B1 in Sterculiae Lychnophorae Semen.To verify the accuracy and reliability of this method,the same batch of samples were simultane-ously determined by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),and the results of the two methods were compared and analyzed.Results:The standard addition method can reduce the loss and interference caused by the sample pretreatment process and effectively offset the matrix effect.The method is accurate and reliable.However,the method also has some shortcomings,such as large sample consumption,high requirements for experimental operation and linear range,making it less suitable for batch analysis,and scenarios with limited sample availability.Conclusion:The standard addition method can effectively overcome the influence of matrix effect.This method can be used as a supplement to the external standard method to effectively improve the accuracy of the determination of aflatoxin B1 content in Sterculiae Lychnophorae Semen,though it still presents certain limitations in terms of operation-al complexity and applicability.关键词
黄曲霉毒素/胖大海/标准加入法/标准曲线法/高效液相-柱后光化学衍生法/超高效液相色谱-串联质谱法/局限性Key words
Aflatoxins/Sterculiae Lychnophorae Semen/Standard addition method/Standard curve method/HPLC-FLD/UPLC-MS/MS/Limitations分类
医药卫生引用本文复制引用
李海亮,刘丽那,李耀磊,王赵,昝珂,金红宇..基于标准加入法对胖大海中黄曲霉毒素B1含量测定的研究[J].中国药物评价,2025,42(6):436-440,5.基金项目
国家重点研发计划项目(2022YFC3501505) （2022YFC3501505）
定西市科技计划项目(DX2024BY003) （DX2024BY003）
