中国医药科学2025，Vol.15Issue(24)：83-88,6.DOI:10.20116/j.issn2095-0616.2025.24.17
儿童自闭症患者外周血中淋巴细胞的TCR多样性特征分析
Analysis of TCR diversity in peripheral blood lymphocytes of children with autism spectrum disorder
摘要
Abstract
Objective To analyze the characteristic T cell receptor(TCR)sequence features of T lymphocytes in children with autism spectrum disorder(ASD)through immune profiling to solve the difficulty of early diagnosis of ASD in children.Methods Screen children with ASD and normal controls who visited the Pediatrics Department of Sichuan Provincial People's Hospital from June 2022 to June 2024 and were aged from 1 to 14 years.Isolate their blood lymphocytes and extract RNA.After reverse transcription amplification,obtain the sequences of the variable regions of TCR through high-throughput sequencing.So as to establish the TCR immune profile and analyze its characteristics.Results A total of 7 participants were included in the ASD group,172 in the healthy control group,and 8 in the cross validation group.Analysis revealed the presence of characteristic high sharing TCR sequences among patients,and the sum of characteristic sequence expression levels showed significant differences between ASD group patients and the healthy control group.The characteristic indices of samples from patients with ASD and those from the healthy control group were analyzed using receiver operating characteristic curve(ROC)analysis.The area under the curve(AUC)of ROC was 0.79.Conclusion There is a significant difference between the TCR repertoire of ASD patients and that of healthy controls,suggesting specific immune responses related to ASD development.This finding may enable non-invasive TCR-based screening and early ASD diagnosis in the future.关键词
儿童自闭症/TCR多样性/高通量测序/特征性TCR序列/早期筛查与诊断Key words
Autism spectrum disorder in children/TCR diversity/High-throughput sequencing/Characteristic TCR sequences/Early screening and diagnosis分类
医药卫生引用本文复制引用
万政伟,卓越,张志新,孙平,孙兆辰,赵娟,赵磊,张金泽,刘玉萍..儿童自闭症患者外周血中淋巴细胞的TCR多样性特征分析[J].中国医药科学,2025,15(24):83-88,6.基金项目
四川省科学技术厅重点研发计划项目(2023YFS0196). （2023YFS0196）
