中国肿瘤生物治疗杂志2025，Vol.32Issue(12)：1247-1252,6.DOI:10.3872/j.issn.1007-385x.2025.12.005
人参皂苷Rg3通过调控PI3K/AKT/mTOR通路介导的磷酸戊糖途径对乳腺癌细胞免疫逃逸的影响
Effect of ginsenoside Rg3 on immune escape of breast cancer cells via regulating pentose phosphate pathway mediated by PI3K/AKT/mTOR signaling pathway
摘要
Abstract
Objective:To investigate the effect of ginsenoside Rg3(GRG3)on the immune escape of breast cancer cells by regulating pentose phosphate pathway(PPP)mediated by PI3K/AKT/mTOR signaling pathway.Methods:MCF7 cells were routinely cultured and divided into five groups:control group,GRG3 low-dose(GRG3-L)group,GRG3 high-dose(GRG3-H)group,GRG3-H+740Y-P(PI3K activator)group,and Ly294002(PI3K inhibitor)group.Colony formation assay,flow cytometry,DCFH-DA fluorescent probe assay,and WB were used to evaluate cell proliferation,apoptosis,ROS levels,and the expression of proteins related to the PPP and the PI3K/Akt/mTOR signaling pathway in each group,respectively.The cytotoxic effect of NK-92MI cells against MCF7 cells was measured using the CCK-8 assay.ELISA was employed to detect the levels of CXCL-2,CXCL-8,G6PD,and NADPH in MCF7 cells,as well as the levels of TNF-α and IFN-γ in the culture supernatant of co-cultured cells.Results:GRG3 significantly suppressed the clonogenic ability of MCF7 cells(all P<0.05),promoted apoptosis of MCF7 cells(all P<0.05),and reduced the levels of CXCL-2,CXCL-8,G6PD,NADPH,and ROS in MCF7 cells(all P<0.05).Additionally,GRG3 inhibited the PPP and PI3K/Akt/mTOR signaling pathways(all P<0.05),promoted the secretion of TNF-α and IFN-γ by NK-92MI cells(all P<0.05),and enhanced the cytotoxicity of NK-92MI cells against MCF7 cells(all P<0.05).These effects were partially reversed by 740Y-P(P<0.05).Conclusion:GRG3 inhibits the biological behavior and immune escape of breast cancer MCF7 cells by suppressing the PI3K/AKT/mTOR pathway-mediated PPP.关键词
乳腺癌/MCF7细胞/NK-92MI细胞/人参皂苷Rg3/PI3K/AKT/mTOR通路/磷酸戊糖途径/免疫逃逸Key words
breast cancer/MCF7 cell/NK-92MI cell/ginsenoside Rg3/PI3K/AKT/mTOR pathway/pentose phosphate pathway/immune escape分类
医药卫生引用本文复制引用
李彬,黄琳..人参皂苷Rg3通过调控PI3K/AKT/mTOR通路介导的磷酸戊糖途径对乳腺癌细胞免疫逃逸的影响[J].中国肿瘤生物治疗杂志,2025,32(12):1247-1252,6.基金项目
武汉市卫健委中医药科研项目(No.WZ24B58) （No.WZ24B58）
