中国中西医结合皮肤性病学杂志2025，Vol.24Issue(6)：493-497,5.
橄榄油干预对脂溢性皮炎模型及COX-2/PGE2/EP轴的影响
Effects of Olive Oil Intervention on Seborrheic Dermatitis Models and the COX-2/PGE2/EP Axis
摘要
Abstract
Objective In order to investigate the effects of application frequency and dosage of exogenous olive oil on the clinical severity of seborrheic dermatitis(SD)and its correlation by the cyclooxygenase-2(COX-2)/prostaglandin E2(PGE2)/prostaglandin E receptor(EP)signaling pathway.Methods A total of 50 SD guinea pig models were divided into five groups using the simple random ranking method:Group A(0.5 mL applied daily),B(1.0 mL applied daily),C(1.0 mL applied every 3.5 days),D(0.5 mL applied every 3.5 days)and E(model control),with 10 animals in each group.Experimental groups were received topical olive oil for two weeks.Intervention efficacy was evaluated through clinical scoring,histopathological assessment and molecular analyses.The pathological changes were observed by hematoxylin-eosin(HE)staining.The protein expressions of COX-2 and PGE2 were detected by enzyme-linked immunosorbent assay(ELISA).Immunohistochemical(IHC)staining was performed to determine the expression profiles of EP subtypes.Results Group B exhibited the highest clinical severity scores compared to group A,C and D(P＜0.01,P＜0.001,P＜0.01).The score of group C was lower than that of group E(P＜0.05).Group B showed marked epidermal hyperkeratosis,acanthosis and inflammatory cells infiltration.The expression levels of COX-2 and EP2 in group A and B were higher than those in group D(P＜0.05,P＜0.01),while the expression level of EP3 in group B was lower than that in group D(P＜0.05).The expression level of EP4 in group B was higher than that in group E(P＜0.05).There was no statistically significant difference in PGE2 and EPl between the groups(P＞0.05).Conclusion Daily topical application of high-dose olive oil(1.0 mL applied daily)may exacerbate SD by upregulating COX-2,EP2 and EP4 expression,which promotes pro-inflammatory responses,coupled with insufficient EP3 expression that attenuates anti-inflammatory effects.关键词
环氧合酶-2/前列腺素E2/前列腺素E受体/橄榄油/脂溢性皮炎Key words
Cyclooxygenase-2/Prostaglandin E2/Prostaglandin E receptor/Olive oil/Seborrheic dermatitis分类
医药卫生引用本文复制引用
裴琴,黄美林,周茂林,于越,何慧琼,王国江,秦海红,陈敏..橄榄油干预对脂溢性皮炎模型及COX-2/PGE2/EP轴的影响[J].中国中西医结合皮肤性病学杂志,2025,24(6):493-497,5.基金项目
上海市卫生健康委员会卫生行业临床研究青年项目(编号:20214Y0350) （编号:20214Y0350）
上海市浦东新区卫生健康委员会青年医学人才培养计划(编号:PWRq2022-24). （编号:PWRq2022-24）
