中国中医骨伤科杂志2026，Vol.34Issue(1)：35-42,8.DOI:10.20085/j.cnki.issn1005-0205.260106
壮腰通络方调节SIRT1/HIF-1α信号通路抑制大鼠髓核细胞衰老延缓椎间盘退变的实验研究
Zhuangyao Tongluo Formula Delays Intervertebral Disc Degeneration by Inhibiting Rat Nucleus Pulposus Cell Senescence via the SIRT1/HIF-1α Signaling Pathway
摘要
Abstract
Objective:Based on the theory of"invigorating the kidney and activating blood circulation",this study aims to investigate the mechanism by which Zhuangyao Tongluo for-mula(ZYTLF)delays intervertebral disc degeneration(IVDD)by inhibiting nucleus pulposus cell(NPC)senescence through regulation of the SIRT1/HIF-1α signaling pathway.Methods:Seventy 8-week-old SPF male rats were randomly divided into six groups:blank group,model group,positive drug control group(Celecoxib),and Zhuangyao Tongluo for-mula low-dose,medium-dose,and high-dose groups.Except for the blank group,a lumbar instability-induced IVDD model was established in all other groups by resection of the spinous processes,articular processes,and supraspinous ligaments.Intragastric administration was initiated on the third day after modeling and continued for 8 weeks.Behavioral perform-ance was observed,and intervertebral disc and nucleus pulposus tissues were collected.Hematoxylin-eosin(HE)staining and Safranin O-Fast Green(SO/FG)staining were used to observe the morphology of the intervertebral disc tissues.Western Blot and real-time quantitative PCR(RT-qPCR)were employed to detect the protein and mRNA expression lev-els of SIRT1,HIF-1α,p16,and Ki-67 in the nucleus pulposus tissues.Results:Compared with the blank group,the nucleus pulposus tissues in the model group exhibited shrinkage and aggregation,disordered arrangement of the annulus fibrosus,and severe loss of matrix proteoglycans.The protein and mRNA expression levels of HIF-1α and p16 were significantly in-creased(P＜0.05 or P＜0.01),while the protein and mRNA expression levels of SIRT1 and Ki-67 were decreased;how-ever,the difference in Ki-67 mRNA expression was not statistically significant(P＞0.05).Compared with the model group,the pathological damage in all ZYTLF intervention groups was alleviated to varying degrees,with the medium-dose group showing the most significant protective effect on disc structure and matrix.The protein and mRNA expression lev-els of HIF-1α and p16 were significantly decreased(P＜0.05 or P＜0.01),and the protein and mRNA expression levels of SIRT1 were significantly increased(P＜0.05 or P＜0.01).There were no statistically significant differences in the protein and mRNA expression levels of Ki-67(P＞0.05).Conclusion:Zhuangyao Tongluo formula can inhibit the senes-cence of rat nucleus pulposus cells and ameliorate intervertebral disc tissue injury,thereby playing a role in delaying IVDD.Its mechanism may be related to the regulation of the SIRT1/HIF-1α signaling pathway.关键词
壮腰通络方/椎间盘退变/衰老/氧化应激Key words
Zhuangyao Tongluo formula/intervertebral disc degeneration/senescence/oxidative stress分类
医药卫生引用本文复制引用
魏佳铭,彭伟,朱立国,赵泽龙,孙凯,秦晓宽,魏戌,李玲慧,尹逊路,杨克新,高春雨..壮腰通络方调节SIRT1/HIF-1α信号通路抑制大鼠髓核细胞衰老延缓椎间盘退变的实验研究[J].中国中医骨伤科杂志,2026,34(1):35-42,8.基金项目
国家自然科学基金青年科学基金项目(82205152)中国中医科学院优秀青年科技人才(创新类)项目(ZZ16-YQ-023)中华中医药学会青年人才托举工程项目(CACM-2023-QNRC2-A06) （82205152）
