浙江大学学报（医学版）2025，Vol.54Issue(6)：737-745,9.DOI:10.3724/zdxbyxb-2025-0602
卵巢组织冷冻保存和移植在青春期前女童中的应用进展
Advances in ovarian tissue cryopreservation and transplanta-tion for fertility preservation in prepubertal patients
摘要
Abstract
Ovarian tissue cryopreservation and transplantation is currently the only feasible method for preserving both fertility and ovarian endocrine function in prepubertal females.It is indicated for those requiring gonadotoxic therapies such as chemotherapy,radiotherapy,or bone marrow transplantation for malignant diseases,as well as for non-malignant diseases,including immunologic,metabolic,and hematologic benign diseases requiring bone marrow transplantation,and other populations at high risk of premature ovarian insufficiency.The procedure involves laparoscopic retrieval of ovarian tissue,followed by slow-programmed cryopreservation.When the primary disease is cured and fertility or hormonal function restoration is desired,the tissue is thawed and transplanted,most commonly to an orthotopic site.For patients at high risk of ovarian malignancy,pre-transplantation assessment of minimal residual disease in the ovarian cortex is performed using histopathology and molecular biology techniques.Globally,while ovarian tissue cryopreservation and transplantation has led to over 300 live births,the majority result from tissue cryopreserved after puberty.Successful restoration of puberty and subsequent live births following transplantation of tissue frozen before puberty,although demonstrated in reported cases,remain less common.This review systematically summarizes recent advances in the indications,current application status,timing and strategies of ovarian tissue cryopreservation and transplantation,risk assessment of tumor cell reintroduction,and clinical outcomes in prepubertal patients.It also discusses the potential value and current challenges of combining this approach with in vitro oocyte maturation techniques,aiming to provide practical references for clinical practice.关键词
生育力保存/青春期前女童/卵巢组织冷冻保存/卵巢移植/卵母细胞体外成熟/青春期诱导/综述Key words
Fertility preservation/Prepubertal girls/Ovarian tissue cryopreservation/Ovarian transplantation/Oocytes in vitro maturation/Puberty induction/Review分类
医药卫生引用本文复制引用
程姣姣,阮祥燕,杜娟,金凤羽,谷牧青..卵巢组织冷冻保存和移植在青春期前女童中的应用进展[J].浙江大学学报（医学版）,2025,54(6):737-745,9.基金项目
首都医科大学附属北京妇产医院/北京妇幼保健院科技创新及转化专项(FCYYJC202409) （FCYYJC202409）
国家外国专家个人类项目(S20240236,S20240230)This study was supported by Science and Technology Innovation and Translation Project of Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing Maternal and Child Health Care Hospital(FCYYJC202409)and National Foreign Experts Individual Project(S20240236,S20240230) （S20240236,S20240230）
