针灸临床杂志2025，Vol.41Issue(12)：65-71,7.DOI:10.19917/j.cnki.1005-0779.025238
夹脊电针对脊髓损伤大鼠程序性坏死相关因子的影响
Effects of Jiaji EA on Programmed Necrosis-Related Factors in Rats with SCI
摘要
Abstract
Objective:To investigate the effects of Jiaji electro-acupuncture(EA)on programmed necrosis-related factors in the spinal cord tissue of rats with spinal cord injury(SCI),and to clarify the mechanism by which Jiaji EA promotes the repair of spinal cord tissue damage.Methods:Allen's SCI model was established using an NYU Impactor M-Ⅲ.36 SD rats were randomly divided into the sham operation group,the model group,and the Jiaji EA group,with 12 rats in each group.Each group was further subdivided into 3-day subgroup(n=6)and 7-day subgroup(n=6).In the 7-day subgroup,BBB scores were assessed at 6 h,1 d,3 d,and 7 d to evaluate hindlimb motor function.In both 3-day and 7-day subgroups,serum samples were collected after anesthesia for ELISA analysis and injured spinal cord tissues were collected for HE staining and qRT-PCR detection.The expressions of programmed necrosis-related factors(RIPK1,RIPK3,MLKL)and necrosis-related inflammatory mediators(HMGB1,IL-6)were analyzed.Results:BBB score analysis showed that the Jiaji EA group had significantly higher scores on both 3 d and 7 d compared with the model group(P＜0.05).HE staining revealed that,relative to the model group,the Jiaji EA group exhibited fewer necrotic cavities in the spinal cord,reduced neuronal swelling,more preserved gray matter structure,less neuronal loss,and alleviated inflammatory cell infiltration.qRT-PCR results showed that on 3 d and 7 d after surgery,the mRNA expressions of programmed necrosis-related factors(RIPK1,RIPK3,MLKL)in spinal cord tissue were significantly higher in both the model group and the Jiaji EA group compared with the sham operation group(P＜0.01).The Jiaji EA group showed significantly lower mRNA expressions of RIPK1,RIPK3,and MLKL compared with the model group after the treatment(P＜0.05).Comparisons between the 7-day and 3-day model subgroups revealed significant differences in mRNA expressions of RIPK3 and MLKL(P＜0.05),and similar significant differences were observed between the 7-day and 3-day Jiaji EA subgroups(P＜0.05).ELISA results showed that the expressions of HMGB1 and IL-6 were significantly higher in the model group than in the sham operation group(P＜0.01).The Jiaji EA group showed significantly reduced expressions of HMGB1 and IL-6 compared with the model group after the treatment(P＜0.05).Significant differences in the expressions of HMGB1 and IL-6 were observed between the 7-day and 3-day subgroups in both the model group and the Jiaji EA group(P＜0.05).Conclusion:Following SCI,necrosis of tissue cells leads to the release of inflammatory factors,with a marked accumulation at 3 days post-injury and a decline by day 7.Jiaji EA has been found to inhibit spinal cord cell necrosis and the release of inflammatory mediators,thereby protecting spinal cord tissue and promoting repair of the injury.关键词
脊髓损伤/夹脊电针/程序性坏死/炎性因子Key words
Spinal cord injury/Jiaji electro-acupuncture/Programmed necrosis/Inflammatory factor分类
医药卫生引用本文复制引用
田洪昭,王亚楠,尹洪娜,伊思锐,胡丽玲,王顺..夹脊电针对脊髓损伤大鼠程序性坏死相关因子的影响[J].针灸临床杂志,2025,41(12):65-71,7.基金项目
国家自然科学基金,编号:81873378、81704181 （）
首批海南省"南海新星"医疗卫生人才平台项目,编号:NHXX-WJW-2023018 （）
黑龙江省中医药管理局科研项目,编号:ZHY2022-120 （）
黑龙江中医药大学基础医学院青年教师科研项目,编号:JCQNZR-06. （）
