浙江农业学报2025，Vol.37Issue(12)：2458-2467,10.DOI:10.3969/j.issn.1004-1524.20241082
两株新型鹅星状病毒的分离鉴定及其致病性
Isolation,identification and pathogenicity of two novel goose astrovirus strains
摘要
Abstract
To determine the etiology of disease outbreaks in goose flocks at two farms in Anhui Province of China,vi-ruses were isolated from two suspected cases of novel goose astrovirus(GAstV)infection collected between June and July 2023(one case involving 70-day-old growing geese),followed by whole-genome sequencing and animal regres-sion assays.The results demonstrated the successful isolation and identification of two novel GAstV strains,which were designated as HR2306/1 and MG-23.Genomic sequence analysis revealed that both strains had a complete ge-nome length of 7175 nucleotides(nt).Their ORF2 sequences exhibited nucleotide and amino acid sequence homol-ogies exceeding 97%compared with strains isolated from 2016 to 2023.The alignment of ORF2 encoding amino acid sequences indicated shared variation sites between these two strains and the strain HR 2110/1(a GAstV strain isola-ted by the authors'team in 2021),suggesting that HR2306/1 and MG-23 might be evolved from HR2110/1.Animal regression assays confirmed the high pathogenicity of both strains in goslings,with clinical manifestations consistent with natural infections.Characteristic urate deposits were observed on kidney surfaces,while histopathological sec-tions and immunohistochemical analysis revealed significant pathological changes and astrovirus antigens,identifying the kidney as a target organ of GAstV.These findings indicated that GAstV could infect 70-day-old growing geese and retain strong pathogenicity in young goslings.关键词
新型鹅星状病毒/育成鹅/分离鉴定/致病性Key words
novel goose astrovirus/growing goose/isolation and identification/pathogenicity分类
农业科技引用本文复制引用
丁莹莹,王晴,王桂军,杨林平,杨庆,张子晨,蔡霖颖,杨康,李琛,刘惠文,鲍光彬..两株新型鹅星状病毒的分离鉴定及其致病性[J].浙江农业学报,2025,37(12):2458-2467,10.基金项目
安徽省家禽产业技术体系项目(2023-2024) （2023-2024）
安徽省高校协同创新项目(GXXT-2022-057) （GXXT-2022-057）
