浙江农业学报2025，Vol.37Issue(12)：2479-2493,15.DOI:10.3969/j.issn.1004-1524.20250031
感染哈维氏弧菌小黄鱼的组织病理学和转录组学分析
Histopathological and transcriptomic analyses of Larimichthys polyactis infected with Vibrio harveyi
摘要
Abstract
To investigate the immune response mechanisms of fish to Vibrio harveyi,this study used small yellow croaker(Larimichthys polyactis)as the material,and transcriptome sequencing and histopathological examination were conducted on the liver,gill filament,and skin after infection with Vibrio harveyi.The results showed that infec-ted fish exhibited scale detachment,skin ulceration,and severe gill congestion.Microscopic examination revealed necrosis of the skin surface,necrosis of gill epithelial cells and structural damage in the gill lamellae,atrophy of liver parenchyma,and vacuolar degeneration of liver cells.Transcriptome analysis indicated the activation of multiple im-mune-related signaling pathways in the liver,gills,and skin of infected fish,including ECM receptor interaction,JAK-STAT signaling pathway,cytokine cytokine receptor interaction,nucleotide-binding oligomerization domain(NOD)-like receptor signaling pathway,RIG-I like receptor signaling pathway,Toll-like receptor signaling pathway,and cytoplasmic DNA sensing pathway.This study elucidates the molecular mechanisms underlying the multi-tissue pathological damage induced by V.harveyi infection in small yellow croaker at the gene and signaling pathway levels,providing a theoretical basis for future research on disease prevention and control strategies.关键词
小黄鱼/哈维氏弧菌/转录组学/组织病理学/免疫通路Key words
Larimichthys polyactis/Vibrio harveyi/transcriptomics/histopathology/immune pathway分类
农业科技引用本文复制引用
黄慧灵,韩明明,潘鑫煜,赵旭东,颉志刚,徐剑斌,楼宝..感染哈维氏弧菌小黄鱼的组织病理学和转录组学分析[J].浙江农业学报,2025,37(12):2479-2493,15.基金项目
宁波市公益性科技计划项目(2021S053) （2021S053）
浙江省科技计划项目(2023C02029) （2023C02029）
