浙江农业学报2025，Vol.37Issue(12)：2494-2503,10.DOI:10.3969/j.issn.1004-1524.20250359
浙江省5株H9N2亚型禽流感病毒的血凝素基因分子特征与遗传进化分析
Molecular characterization and genetic evolutionary analysis of hemagglutinin gene of five H9N2 subtype avian influenza viruses in Zhejiang Province,China
摘要
Abstract
Avian influenza virus(AIV)subtype H9N2 is the most prevalent low pathogenic avian influenza(LPAIV)in avian species in China,posing a serious threat to the farming industry and public health safety due to its persistent antigenic drift and the risk of cross-species transmission.In order to understand the genetic evolutionary characteristics of H9N2 subtype AIV prevalent in Zhejiang Province,the present study was conducted to determine the gene sequence and molecular characterization of the hemagglutinin(HA)gene of five locally isolated H9N2 sub-type AIV strains.The results showed that the five isolates belonged to the h 9.4.2.1 and h9.4.2.5 branches of the Eurasian lineage,and the nucleotide and amino acid homologies of the HA gene ranged from 83.51%to 99.88%and 86.33%to 99.83%,respectively.Notably,the isolates A/Chicken/Zhejiang/HZ01/2022 and A/Duck/Zhejiang/TZ01/2022 showed only 88.24%-88.35%nucleotide homology and 89.43%-90.82%amino acid homology of HA gene with the vaccine strain CK/SS/94.T he HA cleavage sites of all isolates were characterized by single basic amino acid,which was consistent with the characteristics of low pathogenicity virus.Receptor binding site analysis revealed mutations I163T,H191N,A198T/V,Q234L,and Q235M,which might enhance the binding ability of the virus to the human sialic acid α-2,6-galactose receptor.Glycosylation site analysis revealed the presence of 7-8 po-tential glycosylation sites in the isolates,with deletions at sites 218 and 551 and additions at sites 206 and 313,and these mutations may enhance viral adaptation to mammalian hosts.In addition,HA protein antigenic sites were pres-ent with mutations at 234,285 and 334,while sites 181,280,281 and 296 were highly conserved.The study sug-gests that Zhejiang Province H9N2 subtype AIV continues to evolve through mutations at key sites and altered glyco-sylation patterns,which may lead to enhanced virulence,decreased vaccine protection efficacy,and increased risk of cross-species transmission.There is an urgent need to strengthen the molecular surveillance of local endemic strains and optimize the vaccine strain match to enhance the control efficacy and reduce the public health threat.关键词
禽流感病毒/H9N2亚型/HA基因/遗传进化/基因分析Key words
Avian influenza virus/H9N2 subtype/HA gene/genetic evolution/genetic analysis分类
农业科技引用本文复制引用
刘士哲,倪征,张存,屈勇刚,云涛,华炯钢,陈柳,谢荣辉,叶伟成,张传亮,朱寅初,冯肖肖,付媛..浙江省5株H9N2亚型禽流感病毒的血凝素基因分子特征与遗传进化分析[J].浙江农业学报,2025,37(12):2494-2503,10.基金项目
浙江省"三农九方"科技协作计划(2023SNJF059) （2023SNJF059）
浙江省农业重大技术协同推广计划(2025ZDXT17-04) （2025ZDXT17-04）
新疆维吾尔自治区家禽产业技术体系项目(XJARS-12-07,2025xjjq-z-03) （XJARS-12-07,2025xjjq-z-03）
