浙江农业学报2025，Vol.37Issue(12)：2525-2534,10.DOI:10.3969/j.issn.1004-1524.20240757
草莓内生菌的分离筛选及其功能特性
Isolation,screening,and functional characteristics of endophytes from strawberry
解雨佳 1李镇江 2沙飞宇 1张琳 3仝雅娜 4乔长晟 5曹伟锋1
作者信息
- 1. 天津科技大学 生物工程学院,天津 300457
- 2. 天津科技大学 生物工程学院,天津 300457||四川百川金开生物工程有限公司,四川 成都 611130
- 3. 天津慧智百川生物工程有限公司,天津 300456
- 4. 天津市农业科学院 林业果树研究所,天津 300384
- 5. 天津科技大学 生物工程学院,天津 300457||天津慧智百川生物工程有限公司,天津 300456
- 折叠
摘要
Abstract
To explore the various potential functions of strawberry endophytes and provide new strain resources for the subsequent development of endophytic microbial agents,the strawberry cultivar Hongyan was used as the research ob-ject to isolate and screen endophytes.The plate method was employed to determine their growth-promoting abilities,while the five-point confrontation assay was used to evaluate their antagonistic activities against four common straw-berry pathogens,namely Botrytis cinerea,Fusarium oxysporum,Colletotrichum gloeosporioides and Colletotrichum acutatum.High-throughput sequencing was applied to analyze the community structure and diversity of the endo-phytes.The results showed that a total of 68 endophytic strains were isolated.Among them,15 strains had the ability to produce indole-3-acetic acid(IAA),48 strains could produce siderophores,8 strains exhibited inorganic phos-phorus solubilization capacity,23 strains had organic phosphorus solubilization ability,28 strains were capable of producing cellulase,33 strains could produce protease,45 strains possessed nitrogen fixation capacity,and 47 strains could release potassium.The antibacterial test results indicated that 14 endophytic strains showed antagonistic activity against all four pathogenic fungi,and 6 other strains exhibited antagonistic effects against at least one patho-gen.At the phylum level,the endophytic bacterial community of the tested strawberry was mainly composed of Pro-teobacteria and Actinobacteria,while the endophytic fungal community was dominated by Ascomycota.At the genus level,the dominant bacterial genera included Streptomyces,Staphylococcus,and Pseudomonas,and the dominant fungal genus was Monographella.关键词
草莓/内生菌/促生功能/拮抗功能/病原菌Key words
strawberry/endophyte/growth-promoting function/antagonistic function/pathogen分类
农业科技引用本文复制引用
解雨佳,李镇江,沙飞宇,张琳,仝雅娜,乔长晟,曹伟锋..草莓内生菌的分离筛选及其功能特性[J].浙江农业学报,2025,37(12):2525-2534,10.
