浙江农业学报2025，Vol.37Issue(12)：2535-2544,10.DOI:10.3969/j.issn.1004-1524.20241071
小麦根际真菌分离及其抗菌活性
Isolation of rhizosphere fungi from wheat and their antifungal activity
摘要
Abstract
Rizosphere microorganisms often promote plant growth,protect plants from pathogen infection,and their population size is much larger than that of non-rhizosphere microorganisms.Therefore,screening biocontrol strains for root disease management from rhizosphere microorganisms is an effective approach.Wheat crown rot,a soil-borne fungal disease,has become increasingly severe in the major wheat-producing areas of the Huang-Huai Plain in recent years.In this study,the dilution plate method was used to isolate fungi from the wheat rhizosphere.The taxonomic status of the obtained strains was determined based on morphological characteristics and ITS sequence analysis.The antagonistic effect of the isolated strains against the pathogen causing wheat crown rot was evaluated using the plate confrontation assay,and the antifungal spectrum of strains with strong antagonistic activity was further determined.The results showed that a total of 52 strains were isolated,which were classified into 14 species belonging to 6 gene-ra,5 families,4 orders,4 classes,and 2 phyla.The six identified genera and their relative abundances were as fol-lows:Fusarium,50.00%;Aspergillus,13.46%;Cladosporium,13.46%;Penicillium,11.54%;Irpex,7.69%;and Sarocladium,3.85%.Among the isolated strains,28 strains mainly exhibited substrate competition against Fu-sarium pseudograminearum(the pathogen of wheat crown rot),with growth inhibition rates ranging from 17%to 75%.Two strains(designated as W-F-15 and W-F-20)mainly showed antibiotic activity.Between them,Aspergil-lus terreus strain W-F-20 had stronger antibiotic activity,with an inhibition zone width of 15 mm and a growth inhibi-tion rate of 62%.Meanwhile,the strain W-F-20 also exhibited antibiotic activity against 9 other plant pathogenic fungi,showing a broad-spectrum resistance.This study provides theoretical support and basic materials for the fur-ther development and utilization of fungal resources in the control of wheat crown rot.关键词
根际真菌/小麦/微生物多样性/抗菌活性Key words
rhizosphere fungi/wheat/microbial diversity/antifungal activity分类
农业科技引用本文复制引用
宋英俊,蔡怡好,张利霞,吕淑芳,李文文,索晨美,张昊,侯典云,赵杏利..小麦根际真菌分离及其抗菌活性[J].浙江农业学报,2025,37(12):2535-2544,10.基金项目
河南省科技攻关项目(242102110227) （242102110227）
河南省科技研发计划联合基金项目(222103810057) （222103810057）
