浙江农业学报2025，Vol.37Issue(12)：2545-2553,9.DOI:10.3969/j.issn.1004-1524.20241094
青海省海东市设施番茄根结线虫病原鉴定
Identification of Meloidogyne spp.infecting tomato in Haidong City,Qinghai Province,China
摘要
Abstract
To identify the root-knot nematode species(Meloidogyne spp.)infecting tomato in greenhouses of the Nongxin Planting Cooperative in Ping'an District,Haidong City,Qinghai Province,this study combined morphologi-cal and molecular biological methods for species identification and evaluated its pathogenicity on tomato using indoor pot inoculation tests.The results showed that the morphometric values and perineal patterns of the female adults were consistent with those of Meloidogyne arenaria.PCR amplification with the species-specific SCAR markers Far/Rar produced a specific band of 420 bp.Phylogenetic analysis based on the rDNA ITS and 28S rRNA D2A/D3B regions revealed that the nematode population clustered in a clade with other M.arenaria sequences,showing more than 95%homology.Based on the integrated morphological and molecular data,the pathogen was identified as M.arena-ria.Inoculated tomato plants exhibited stunted growth,thin stems,and yellowing leaves.Thirty days after inocula-tion,distinct root galls were observed,and the re-isolated nematodes were confirmed to be M.arenaria.This study reports the first identification and documentation of M.arenaria in Qinghai Province,providing a scientific basis for the management of tomato root-knot nematode disease in this region.关键词
番茄/根结线虫/花生根结线虫/病原/致病性Key words
tomato/Meloidogyne spp./Meloidogyne arenaria/pathogen/pathogenicity分类
农业科技引用本文复制引用
鲍梦楠,李劲斌,张宪,闫佳会..青海省海东市设施番茄根结线虫病原鉴定[J].浙江农业学报,2025,37(12):2545-2553,9.基金项目
青海省中央引导地方科技发展资金项目(2024ZY025) （2024ZY025）
