植物保护学报2025，Vol.52Issue(6)：1379-1391,13.DOI:10.13802/j.cnki.zwbhxb.2025.2025033
链格孢菌橘致病型侵染柑橘抗感品种的转录组差异表达分析
Transcriptome differential expression analysis of the tangerine pathotype of Alternaria alternata after infecting citrus resistant/susceptible varieties
摘要
Abstract
To explore the differences in gene expression of the tangerine pathotype of Alternaria alter-nata during the infection of citrus resistant/susceptible varieties,the susceptible variety Hongju and the resistant variety Newhall navel orange were inoculated with mycelial pellets of the tangerine pathotype of A.alternata,respectively.The high-throughput sequencing technology was used to sequence and assemble the transcriptomes of samples at the mycelial stage of the pathogen(0 h)and during three infection time points(12,24,and 48 hours post-inoculation).Additionally,functional annotation and classification were performed,along with differential gene screening and analysis.The results showed that the functions of the specifically differentially expressed genes(DEGs)of the tangerine pathotype of A.alternata after infecting Hongju were mainly enriched in cellular energy metabolism,the synthesis and decomposition of amino acids and coenzymes,and the pectin degradation process.The functions of the DEGs of this pathogen after infecting Newhall navel orange were mainly enriched in cell structure construction,cell communication,and environmental stress response.These results indicated the differ-ences in the biological responses and metabolic pathways activated by the tangerine pathotype of A.alternata when infecting Hongju and Newhall navel orange.When the tangerine pathotype of A.alter-nata was inoculated onto detached leaves of Hongju tangerine,it actively infects the host by coordi-nating the regulation of virulence,metabolism,growth and development processes,thereby suppress-ing the defense response and achieving efficient parasitism adaptation within the host.When the tan-gerine pathotype of A.alternata was inoculated onto detached leaves of Newhall navel orange,the pathogen and the host's defense systems remained in a state of constant interaction.Due to the con-tinuous defense interference from the host,the pathogen was unable to completely break down the host's resistance,this results in the inability of the mycelium to successfully infect and colonize.The real-time quantitatire PCR verification results were consistent with the transcriptional sequencing results,indicating that the transcriptome sequencing data was reliable.关键词
链格孢菌橘致病型/抗感品种/转录组/差异表达基因Key words
the tangerine pathotype of Alternaria alternata/resistant/susceptible varieties/transcrip-tome/differentially expressed gene引用本文复制引用
钟胤珊,张懿丹,唐科志,周常勇..链格孢菌橘致病型侵染柑橘抗感品种的转录组差异表达分析[J].植物保护学报,2025,52(6):1379-1391,13.基金项目
国家现代农业产业技术体系专项(CARS-26),中央高校基本科研业务费专项资金(SWU224007) （CARS-26）
