植物保护学报2025，Vol.52Issue(6)：1392-1403,12.DOI:10.13802/j.cnki.zwbhxb.2025.2025068
番茄品种对细菌性溃疡病的抗性及其抗病相关指标分析
Evaluation of resistance in tomato varieties to bacterial canker and analysis of disease resistance-related indicators
摘要
Abstract
In order to clarify the resistance levels of different tomato varieties to bacterial canker,iden-tify resistant tomato varieties,and elucidate the underlying resistance mechanisms,a needle-puncture inoculation method was used to evaluate the resistance of 66 tomato varieties to this disease.The activi-ties of antioxidant enzymes,malondialdehyde(MDA)content,and the expression levels of disease resistance-related genes were measured in highly resistant and highly susceptible tomato varieties after inoculation with Clavibacter michiganensis,the pathogen causing tomato bacterial canker.The results showed that the resistance of the 66 tomato varieties to bacterial canker could be classified into five grades:Micro Tom was highly resistant,Red Brandy resistant,23 varieties including Fushan 99,Bail-ingqianxi,and Xiaoqianjinhuangfei moderately resistant,27 varieties including Provence,Niuxin Tomato,and Heimeiren susceptible,and 14 varieties including Yellow Pear,Micai Tomato,and Hezuo 909 highly susceptible.Based on the manifestation of resistance,the tested tomato varieties were divided into three types:21 varieties were resistant to pathogen invasion,12 varieties resistant to patho-gen expansion,and 33 varieties dual-sensitive.The activities of peroxidase(POD),superoxide dis-mutase(SOD),and catalase(CAT)in the highly resistant variety Micro Tom inoculated with C.michi-ganensis were significantly higher than those in the highly susceptible variety Hezuo 909.At 72 hours post-inoculation,the POD and CAT activities of the highly resistant variety were 2.28 times and 1.40 times those of the highly susceptible variety,respectively.At 120 hours post-inoculation,the SOD activity of the highly resistant variety was 1.72 times that of the highly susceptible variety,while the MDA content of the highly resistant variety was significantly lower than that of the highly susceptible variety.Real-time quantitative RCR analysis indicated that infection by C.michiganensis induced sig-nificant upregulation of the pathogenesis-related protein-encoding genes LePOD and PR-NP24 in the highly resistant variety.These findings demonstrate that the resistance of tomato varieties to C.michi-ganensis is closely associated with their antioxidant capacity,membrane lipid peroxidation level,and the expression levels of pathogenesis-related genes.关键词
番茄细菌性溃疡病/密执安棒杆菌/品种/抗性评价/抗性指标Key words
tomato bacterial canker/Clavibacter michiganensis/variety/resistance evaluation/resis-tance index引用本文复制引用
张舒涵,刘朋飞,仇智灵,楼兵干..番茄品种对细菌性溃疡病的抗性及其抗病相关指标分析[J].植物保护学报,2025,52(6):1392-1403,12.基金项目
浙江省"尖兵""领雁"研发攻关计划项目(2023C04012) （2023C04012）
