中药药理与临床2025，Vol.41Issue(12)：59-64,6.
雷公藤多苷调控AMPK/mTOR信号通路介导的自噬诱导小鼠精原细胞损伤
Autophagy-Mediated Damage in Mouse Spermatogonia Induced by Tripterygium Glycosides via Regulation of AMPK/mTOR Signaling Pathway
摘要
Abstract
Objective:To explore the mechanism of damage induced by tripterygium glycosides(TG)on mouse spermatogonia.Methods:GC-1 spg cells were divided into the following groups:10％normal serum control group,TG-containing serum groups(5％,10％,and 20％),AMPK pathway inhibitor group,and AMPK pathway inhibitor+TG-containing serum group.The proliferation of GC-1 spg cells was detected using the CCK8 method,and the apoptosis of GC-1 spg cells was detected by TUNEL fluorescence.The expression levels of proteins and mRNAs re-lated to the AMPK-mTOR pathway,autophagy,and apoptosis were detected using Western Blot and RT-PCR.Autophagosomes and autolyso-somes were observed via electron microscopy.Results:Compared with that of the normal serum control group,cell viability of the 5％,10％,and 20％TG-containing serum groups was significantly decreased(P＜0.05),and that of the AMPK pathway inhibitor group was decreased(P＜0.05).The number of apoptotic cells increased gradually with the increase of TG concentration(P＜0.05),and that of the AMPK path-way inhibitor group increased significantly(P＜0.05).The protein and mRNA expression levels of p-AMPK,Beclin1,Atg5,and Bax in TG-containing serum groups were progressively upregulated with the increase of TG concentration(P＜0.05),while those of p-mTOR,p62,and Bcl-2 were gradually downregulated(P＜0.05).In the AMPK pathway inhibitor group,the protein and mRNA expression levels of p-AMPK,Beclin1,Atg5,and Bcl-2 were downregulated(P＜0.05),while those of p-mTOR,p62,and Bax were upregulated(P＜0.05).The number of autophagosomes and autolysosomes increased in all TG-containing serum groups(5％,10％.and 20％)but decreased in the AMPK pathway inhibitor group.Compared with the AMPK inhibitor group,the AMPK pathway inhibitor+20％TG-containing serum group showed signifi-cantly higher cell viability(P＜0.05),reduced number of apoptotic cells(P＜0.05),upregulated protein and mRNA expression of p-AMPK,Beclin1,Atg5,and Bcl-2(P＜0.05),and downregulated expression of p-mTOR,p62,and Bax(P＜0.05).The number of autophagosomes and autolysosomes also increased in this group.Conclusion:TG may cause excessive autophagy in mouse spermatogonia by activating the AMPK/mTOR pathway,resulting in damage to GC-1 spg spermatogonia.关键词
雷公藤多苷/腺苷单磷酸活化蛋白激酶/哺乳动物雷帕霉素靶蛋白通路/小鼠精原细胞/自噬Key words
Tripterygium glycoside/AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)pathway/Mouse sper-matogonia/Autophagy引用本文复制引用
韩姗姗,黄欣,丁樱,任献青,张霞,代彦林,徐闪闪,程齐..雷公藤多苷调控AMPK/mTOR信号通路介导的自噬诱导小鼠精原细胞损伤[J].中药药理与临床,2025,41(12):59-64,6.基金项目
中国博士后科学基金项目(编号:2021M690940). （编号:2021M690940）
