中药药理与临床2025，Vol.41Issue(12)：65-69,5.
姜黄素调控miR-134-5p/ABCG2通路对肝癌HepG2细胞增殖和凋亡的影响
Curcumin Regulates miR-134-5p/ABCG2 Pathway to Inhibit Proliferation and Promote Apoptosis of Hepatocellular Carcinoma HepG2 Cells
摘要
Abstract
Objective:To explore the effects of curcumin on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells by modu-lating the miR-134-5p/ATP-binding cassette G2(ABCG2)signaling pathway.Methods:Human hepatocellular carcinoma HepG2 cells were treated with different concentrations of curcumin,and then the viability of HepG2 cells was detected by the CCK-8 assay and the half maximal inhibitory concentration(IC50)was calculated.The existence of binding sites between miR-134-5p and ABCG2 was predicted by TargetScan,an online target gene prediction website.The dual luciferase gene reporter assay was employed to verify the targeting relationship between miR-134-5p and Abcg2.HepG2 cells were randomly allocated into blank control,negative control,model control,and curcumin groups,and the cells were transfected with lipofectamine 3000.qRT-PCR was conducted to determine the expression levels of miR-134-5p and Abcg2 mRNA in each group.The EdU method was used to detect the cell proliferation in each group,and flow cytometry was employed to detect the cell cy-cle and the apoptosis rate.The cellular mitochondrial membrane potential was measured by the JC-1 assay,and Western blot was employed to determine the protein levels of ABCG2 and apoptosis markers B-cell lymphoma(Bcl-2),Bcl-2-associated X protein(Bax),and cysteinyl as-partate-specific proteinase 3(caspase-3)in cells.Results:Compared with 0 μmol/L,curcumin at concentrations of 60-100 μmol/L de-creased the viability of HepG2 cells(P＜0.01),with the IC50 of 60.08 μmol/L.The results of dual luciferase reporter assay showed that Ab-cg2 was the target gene of miR-134-5p.Compared with the blank and negative control groups,the model control group presented down-regula-tion in miR-134-5p expression(P＜0.01),up-regulation in the mRNA level of Abcg2(P＜0.01),an increase in cell proliferation rate(P＜0.01),reductions in apoptosis rate(P＜0.01)and the ratio of G0/G1 phase cells(P＜0.01),and an elevation in cellular mitochondrial mem-brane potential(P＜0.01).In addition,the model control group showcased down-regulated protein levels of Bax and caspase-3(P＜0.01)and up-regulated protein levels of ABCG2 and Bcl-2(P＜0.05 or P＜0.01).Compared with the model control group,the curcumin treatment up-regulated the miR-134-5p expression(P＜0.01),down-regulated the mRNA level of Abcg2(P＜0.01),decreased the cell proliferation rate(P＜0.01),increased the cell apoptosis rate(P＜0.01)and the ratio of G0/G1 phase cells(P＜0.01),reduced the proportion of cells in S phase(P＜0.01),and decreased the cellular mitochondrial membrane potential(P＜0.01).In addition,curcumin up-regulated the protein levels of Bax and caspase-3(P＜0.01)and down-regulated the protein levels of ABCG2 and Bcl-2(P＜0.05 or P＜0.01).Conclusion:Cur-cumin inhibits the proliferation and promotes the apoptosis of HepG2 cells by regulating the miR-134-5p/ABCG2 signaling pathway.关键词
姜黄素/miR-134-5p/乳腺癌抵抗蛋白/肝癌HepG2细胞/增殖/凋亡Key words
Curcumin/miR-134-5p/ABCG2/Hepatocellular carcinoma HepG2 cells/Proliferation/Apoptosis引用本文复制引用
廖昭辉,谢正元..姜黄素调控miR-134-5p/ABCG2通路对肝癌HepG2细胞增殖和凋亡的影响[J].中药药理与临床,2025,41(12):65-69,5.基金项目
江西省教育厅科学技术研究项目(编号:GJJ200193). （编号:GJJ200193）
