中药药理与临床2025，Vol.41Issue(12)：70-75,58,7.
基于脂质组学探讨何首乌致特异质肝损伤的潜在机制
Lipidomics Reveals Potential Mechanism of Heshouwu-Induced Idiosyncratic Liver Injury
摘要
Abstract
Objective:To explore the synergistic effect of Heshouwu(何首乌)and the susceptible factor tumor necrosis factor(TNF)-α on liver injury and its effect on lipid metabolism.Methods;C57 mice were randomly allocated into blank,Heshouwu(3.12 g/kg and 6.24 g/kg),TNF-α(10 ng/g),TNF-α(10 ng/g)+Heshouwu(3.12 g/kg or 6.24 g/kg)groups.The liver tissue and plasma samples of mice in each group were collected,and the histopathological changes were observed.The levels of liver injury markers alanine aminotransferase(ALT),as-partate aminotransferase(AST),alkaline phosphatase(ALP),direct bilirubin(DBIL)and pro-inflammatory cytokines interleukin(IL)-6 and IL-1β in the plasma were determined.Plasma lipidomics based on ultra-high performance liquid chromatography-mass spectrometry was employed to analyze changes in lipid metabolism.Results:Compared with the blank group,the TNF-α+Heshouwu(3.12 g/kg)and TNF-α+Heshouwu(6.24 g/kg)groups showed expansion of the portal area and obvious inflammatory infiltration in the liver tissue and raised levels of ALT,AST,ALP,IL-6,and IL-1β(P＜0.05,P＜0.01).Lipidomics results showed that compared with the blank group,TNF-α+Heshouwu(6.24 g/kg)led to obvious abnormalities in lipid metabolism,which were manifested as elevated levels of phosphatidylcholine,phosphatidyle-thanolamine,phosphatidylglycerol,phosphatidylserine,partial triglycerides,diglycerides,sphingomyelin,ceramide,hexaglycosylceramide,zy-mosterol,and coenzyme.Conclusion:Heshouwu combined with TNF-α can lead to liver injury in mice by regulating lipid metabolism and ag-gravating inflammatory responses.关键词
何首乌/特异质型药物性肝损伤/脂质组学/肿瘤坏死因子α/协同致毒Key words
Heshouwu(何首乌)/Idiosyncratic drug-induced liver injury/Lipidomics/Tumor necrosis factor-α/Synergistic toxicity引用本文复制引用
白万权,迪丽热巴·艾麦尔,顾健,王伽伯,张乐..基于脂质组学探讨何首乌致特异质肝损伤的潜在机制[J].中药药理与临床,2025,41(12):70-75,58,7.基金项目
国家自然科学基金青年科学基金项目(编号:82204752) （编号:82204752）
西南民族大学引进人才科研启动金资助项目(编号:RQD2021057) （编号:RQD2021057）
西南民族大学中央高校基本科研业务费专项(编号:ZYN2023082). （编号:ZYN2023082）
