中医药学报2026，Vol.54Issue(1)：26-32,7.DOI:10.19664/j.cnki.1002-2392.260005
基于肝脏脂质代谢紊乱的白鲜皮水提液致大鼠肝毒性研究
Study on Hepatotoxicity of Dictamni Cortex Water Extract in Rats Based on Liver Lipid Metabolism Disorder
摘要
Abstract
Objective:To explore the mechanism of liver injury induced by Dictamni Cortex water extract through interference with lipid metabolism in the liver.Methods:Thirty-two SPF-grade male SD rats were randomly divided into four groups:control group,high-dose Dictamni Cortex group(8.1 g/kg),medium-dose group(2.7 g/kg),and low-dose group(0.9 g/kg),with 8 rats in each group.The rats were administered by gavage for 8 weeks.Liver weight,liver-to-body weight ratio,and liver toxicity-related indicators were measured to observe changes in the rats·liver.Hematoxylin-eosin(HE)staining and Oil Red O staining were used to assess the pathological changes and lipid droplet accumulation in liver tissues.The mRNA levels of AMPK,PPAR-α,IL-1β,and TNF-α were detected in liver tissues.Lipid levels in the liver were analyzed by LC-MS/MS,and multivariate statistical analysis was used to identify differentially expressed lipid metabolites.Results:Compared with the control group,the liver-to-body weight ratio was increased in all Dictamni Cortex dose groups(P<0.01,P<0.001).In the high-dose group,AST、ALT、ALB levels were significantly increased(P<0.05,P<0.001).HE and Oil Red O staining showed that the high-dose group significantly induced fatty liver lesions in rats.The mRNA levels of inflammatory factors IL-1β and TNF-α were significantly increased(P<0.01),while the mRNA levels of PPAR-α,were significantly reduced(P<0.01).Lipidomics analysis showed that,compared to the control group,156 lipid species were significantly altered in the high-dose group,including glycerolipids,phosphatidylcholine,sphingolipids,etc.Specific lipids such as PC(16:0/18:2),PC(16:0/18:1)、PC(14:0/18:2)、PC(15:0/18:2)、TG(16:0/16:0/16:0),TG(18:0/16:0/16:0)、SM(d42:1),SM(d42:2),SM(d42:3)showed significantly variation levels after treatment.These results suggest that the high-dose Dictamni Cortex water extract disturbed lipid metabolism in the liver of rats.Conclusion:The water extract of Dictamni Cortex induces liver injury in rats by interfering with lipid metabolism,particularly sphingolipids(SM),ceramides(Cer),triglycerides(TG),and phosphatidylcholine(PC).关键词
白鲜皮/脂质组学/肝损伤Key words
Dictamni Cortex/Lipidomics/Liver injury分类
医药卫生引用本文复制引用
高蕊,卢芳,孙慧娟,张萌萌,邓戈宇,黄琳,刘树民..基于肝脏脂质代谢紊乱的白鲜皮水提液致大鼠肝毒性研究[J].中医药学报,2026,54(1):26-32,7.基金项目
国家重点研发计划-中医药现代化(2022YFC3502100,2022YFC3502102,2022YFC3502102-04) （2022YFC3502100,2022YFC3502102,2022YFC3502102-04）
