中医药学报2026，Vol.54Issue(1)：33-39,7.DOI:10.19664/j.cnki.1002-2392.260006
基于间接竞争性酶联免疫吸附法研究枳实炭碳点对芍药苷的药代动力学影响
Pharmacokinetics of Paeoniflorin Influenced by Aurantii Fructus Carbon Quantum Dots Based on Indirect Competitive Enzyme-Linked Immunosorbent Assay
摘要
Abstract
Objective:To investigate the pharmacokinetic effects of Aurantii Fructus Carbon Quantum Dots(AFIC-CDs)on paeoniflorin(PF),the main active component of Paeonia.Methods:Male Kunming mice were randomly divided into three groups:high-dose AFIC-CDs(120 mg·mL-1)and PF co-administration group(H),low-dose AFIC-CDs(60 mg·mL-1)and PF co-administration group(L),and PF alone(2.0 mg·mL-1)group(S).Mice were orally administered mixed solutions of high or low-dose AFIC-CDs with the same dose of PF,or PF alone.Paeoniflorin levels in the mouse blood were determined by indirect competitive enzyme-linked immunosorbent assay(icELISA)using anti-PF monoclonal antibodies,and pharmacokinetic parameters of PF were compared among the groups.Results:Co-administration of AFIC-CDs with PF significantly increased PF blood concentration and the area under the blood concentration-time curve(AUC-t)(P<0.05,P<0.01),with increases observed as the AFIC-CDs concentration increased.Compared to PF administration alone,the Cmax of PF in the high-dose and low-dose AFIC-CDs+PF groups increased from(695.43±229.73)ng·mL-1 to(1 615.31±245.16)ng·mL-1 and(973.11±155.90)ng·mL-1,respectively.The AUC-t increased from(90181.22±20926.42)ng·min·mL-1 to(257 510.74±26 729.64)ng·min·mL-1 and(116 654.67±11 770.02)ng·min·mL-1,respectively.Conclusion:AFIC-CDs can enhance the bioavailability of PF,providing scientific evidence for clinical co-medication to improve therapeutic efficacy.关键词
枳实炭碳点/芍药苷/间接竞争性酶联免疫吸附法/药代动力学/生物利用度/药物相互作用/纳米药物Key words
Aurantii fructus carbon quantum dots/Paeoniflorin/Indirect competitive enzyme-linked immunosorbent assay/Pharmacokinetics/Bioavailability/Drug interaction/Nanomedicine分类
医药卫生引用本文复制引用
李梦含,王苏娜,邹鹏,黄燕,何晨昕,马华根,赵琰,孔慧..基于间接竞争性酶联免疫吸附法研究枳实炭碳点对芍药苷的药代动力学影响[J].中医药学报,2026,54(1):33-39,7.基金项目
北京市自然科学基金-海淀原始创新联合基金资助项目(L222132) （L222132）
中央高校基本科研业务费专项资金项目(2024-JYB-JBZD-040) （2024-JYB-JBZD-040）
