中医药信息2026，Vol.43Issue(2)：31-38,8.DOI:10.19656/j.cnki.1002-2406.20260206
芍药甘草汤调控血浆外泌体miR-216a-5p干预脊髓损伤后肌痉挛的研究
Study on the Intervention of Shaoyao Gancao Decoction in Muscle Spasm after Spinal Cord Injury by Regulating Plasma Exosomal miR-216a-5p
摘要
Abstract
Objective To investigate the mechanism by which Shaoyao Gancao Decoction(SGD)intervenes spasm in spinal-cord-injured(SCI)rats.Methods ① Forty male SD rats were randomly assigned to four groups(n=10 each):Normal,Model,SGD and Positive.Except for the Normal group,rats in other groups received spinal cord hemisection at T9-10 to establish the SCI model.The third day after surgery,rats in Normal and Model groups were gavaged with de-ionized water;Rats in SGD group were gavaged with SGD(at the dose of 3.3 g/kg),and rats in the Positive group were gavaged with baclofen(at the dose of 1.35 mg/kg).The treatment lasted for 2 weeks.Drug efficacy was evaluated with the Basso-Beattie-Bresnahan(BBB)locomotor score.HMGB1 protein in spinal cord homogenates and expression of miR-216a-5p levels were quantified.The regulatory relationship between miR-216a-5p and HMGB1 was further validated by a dual-luciferase reporter gene assay.②Spinal-cord neurons were cultured in vitro,the effect of HMGB1 expression by SGD and miR-216a-5p mimic/inhibitor were assessed.③ Fifty additional SD rats were divided into Model,SGD,miR-216a-5p mimics,miR-216a-5p inhibitor,and exosome groups(n=10 each)to observe HMGB1 expression after the respective interventions.Results ① After 1 and 2 weeks of treatment,BBB scores in the SGD group were markedly higher than in the Model group(P<0.01),exhibited comparable efficacy to the Positive group.The level of HMGB1 increased after the SCI.After the treatment,the HMGB1 decreased in spinal cord tissue,meanwhile the miR-216a-5p expression in the plasma exosome increased.Dual-luciferase reporter gene assay confirmed that the HMGB1 was the target gene of miR-216a-5p.② In cultured neurons in vitro,HMGB1 was up-regulated in the Model group;HMGB1 down-regulated after the SGD and miR-216a-5p mimic transfection,while the miR-216a-5p inhibitor played a promotion role in the expression of HMGB1.③Further animal experiments confirmed that miR-216a-5p from plasma exosomes in the SGD group inhibited the expression of HMGB1 in spinal cord tissue.Conclusion SGD exerts a positive effect on neural repair in SCI rats by downregulating miR-216a-5p in plasma-derived exosomes,which consequently suppresses the expression of HMGB1 in spinal cord tissue.关键词
芍药甘草汤/脊髓损伤/肌痉挛/miR-216a-5p/高迁移率族蛋白B1Key words
Shaoyao Gancao Decoction/Spinal cord injury/Muscle spasm/MiR-216a-5p/HMGB1引用本文复制引用
段小波,郭珂珂,郭云鹏,谢艳,周英杰,岳宗进..芍药甘草汤调控血浆外泌体miR-216a-5p干预脊髓损伤后肌痉挛的研究[J].中医药信息,2026,43(2):31-38,8.基金项目
河南省医学科技攻关省部共建项目(SBGJ202102075) （SBGJ202102075）
河南省青苗人才项目(豫卫中医药科教[2024]4号) （豫卫中医药科教[2024]4号）
洛阳市公益性行业医疗卫生专项项目(2022007A) （2022007A）
