中医药信息2026，Vol.43Issue(2)：47-52,6.DOI:10.19656/j.cnki.1002-2406.20260208
膝痹方对膝骨关节炎血瘀证大鼠软骨的保护作用
Experimental Study of the Cartilage-protective Efforts of Xibi Decoction in the Rats with Knee Osteoarthritis by Syndrome of Blood Stasis
摘要
Abstract
Objective To investigate the cartilage-protective effects of Xibi Decoction in rats with knee osteoarthritis by syndrome of blood stasis(KOA-BS).Methods The syndrome of blood stasis model was induced through adrenalin injections and ice-water stress method;The knee osteoarthritis model was induced through intra-articular injections of papain and L-cysteine.After modeling,the rats were randomly divided into four groups:model group,positive group,Xibi Decoction high-dose group and Xibi Decoction low-dose group,with eight rats in each group.Another 8 rats were selected as the normal group,without any modeling intervention,and only injected and gavaged with the equal volume of physiological saline.The model group and normal group were gavaged with the equal volume of physiological saline.According to the rats'body surface area,the Xibi Decoction high-dose group were gavaged with 50 g/kg of Xibi Decoction,while the Xibi Decoction low-dose group were gavaged with 25 g/kg of Xibi Decoction,the positive group were gavaged with 50 mg/kg of diacerein.Each group were administered once per day for six weeks.The overall psychical signs of the rats in each group were monitored and recorded before and after the treatment.Knee joint swelling were measured before treatment and at 2,4 and 6 weeks post-treatment.Blood rheology indicators were assessed including the plasma viscosity,whole blood viscosity,and erythrocyte aggregation index.Histopathological evaluations of articular cartilage were conducted through HE staining and scored by Mankin's scoring system.Additionally,gene expression levels of collagen type Ⅱ α1 chain(CⅡα1),aggrecan(Acan),and Cartilage Oligomeric Matrix Protein(Comp)were analyzed by the RT-qPCR method.Results The rats in the high-and low-dose groups showed improvement in knee osteoarthritis by syndrome of blood stasis,with significant reductions in plasma viscosity,whole blood viscosity,and erythrocyte aggregation index compared with the model group(P<0.01,P<0.05).Knee joint swelling was significantly reduced in the high-dose group after 4 and 6 weeks of treatment(P<0.05,P<0.01),and in the low-dose group,the knee joint swelling was significantly reduced after 6 weeks(P<0.01).The rats in high-and low-dose groups exhibited improvements in the flatness of the articular cartilage surface,structural integrity,and arrangement of chondrocytes,with Mankin's scores significantly lower(P<0.01,P<0.05).Additionally,both treatment groups significantly upregulated the relative gene expression of articular cartilage CIIα1,Acan,and Comp(P<0.01).Compare with the positive group,the relative gene expression levels of CIIα1 and Acan were significantly reduced in the low-dose group(P<0.05).Conclusion Xibi Decoction demonstrates efficacy in ameliorating the overall clinical signs and knee joint symptoms in KOA-BS rats.It also promotes the upregulation of genes related to cartilage anabolism,thereby contributing to cartilage protection.关键词
膝痹方/膝骨关节炎/血瘀证/大鼠/软骨保护Key words
Xibi Decoction/Knee osteoarthritis/Syndrome of blood stasis/Rat/Cartilage protection引用本文复制引用
张上,杨军,冯丽娜,刘炳君..膝痹方对膝骨关节炎血瘀证大鼠软骨的保护作用[J].中医药信息,2026,43(2):47-52,6.基金项目
河南省中医药科学研究专项课题项目(24ZY1013) （24ZY1013）
