安徽农学通报2026，Vol.32Issue(2)：19-23,5.DOI:10.16377/j.cnki.issn1007-7731.2026.02.007
氧化石墨烯调控甘蓝型油菜耐旱性机制
Mechanisms of graphene oxide regulating drought tolerance of Brassica napus
摘要
Abstract
To investigate the regulatory mechanism of graphene oxide(GO)on drought resistance in Brassica napus,this study utilized the cultivar Huayouza 50 as experimental material.Drought stress was applied followed by exogenous treatments with clean water(CK),15 and 25 mg/L GO.The activities of protective enzymes such as superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and ascorbate peroxidase(APX)were measured,along with the contents of osmoregulatory substances including proline(Pro)and soluble protein.Additionally,the content of malondialdehyde(MDA),and the levels of hormones such as gibberellin(GA),indole-3-acetic acid(IAA),and abscisic acid(ABA)were determined in various treatments of B.napus.Furthermore,the transcriptional levels of genes related to GA,IAA,and ABA(RGA,GA3,ARF2,and NCED)were detected using quantitative polymerase chain reaction(qPCR)technology.The results showed that the survival rate of rapeseed treated with 25 mg/L GO was significantly higher than that of the control(31.67%)under drought stress.At the physiological level,compared with CK,the activities of POD,SOD,CAT,and APX in plants were enhanced under 25 mg/L GO treatment,while the MDA content(6.82 μg/g)decreased and proline(623.51 μg/g)and soluble protein(4 939.27 μg/g)accumulated.At the molecular level,compared with CK,25 mg/L GO application upregulated the expression of GA biosynthesis gene GA3 and IAA signaling gene IAA2,while 15 mg/L GO treatment promoted the transcription of NCED,a pivotal gene in ABA synthesis.These findings suggest that GO enhances drought tolerance in B.napus by modulating hormone-related signaling pathways,thereby strengthening antioxidant defense systems and osmotic adjustment capacity.This study provides critical insights into the application of nanomaterials in improving crop stress resistance and highlights the potential of GO as a novel agrochemical agent for sustainable agriculture under abiotic stress conditions.关键词
氧化石墨烯/甘蓝型油菜/耐旱性/生理机制Key words
graphene oxide/Brassica napus/drought tolerance/physiological mechanism分类
农业科技引用本文复制引用
张慧姝,章子爽,刘永杰,张立平,许本波..氧化石墨烯调控甘蓝型油菜耐旱性机制[J].安徽农学通报,2026,32(2):19-23,5.基金项目
湖北省农业农村厅项目"科技服务油菜产业链'515'行动"(鄂农油[2022]7号). （鄂农油[2022]7号）
