安徽中医药大学学报2026，Vol.45Issue(1)：35-41,7.DOI:10.3969/j.issn.2095-7246.2026.01.009
电针通过调控RCAN1促进脑缺血小鼠血管新生与运动功能恢复
Electroacupuncture Promotes Angiogenesis and Motor Functional Recovery in Mice with Cerebra Ischemia by Regulating Regulator of Calcineurin 1
摘要
Abstract
Objective To observe the effect of electroacupuncture at Baihui and Dazhui points on the expression of regulator of calcineurin 1(RCAN1)in the peri-infarct area of a mouse model of cerebral ischemia,and to investigate whether RCAN1 medi-ates the role of electroacupuncture in promoting angiogenesis and improving motor function.Methods Male C57BL/6 mice were randomly divided into normal group,model group,electroacupuncture group,and knockdown+electroacupuncture group,with 6 mice in each group.The method of photochemical thrombosis was used to establish a model of cerebral ischemia.The pole test and the front paw grip strength test were used to assess motor function;TTC staining was used to measure in-farct area;quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of RCAN1;super-resolution microscopy was used to measure vascular density and ratio;ELISA was used to measure the levels of the inflammatory factors interleukin-1β(IL-1β)and interleukin-6(IL-6).Results Compared with the normal group,the model group had significant increases in pole climbing time and retention time(P＜0.05),a significant reduction in front paw grip score(P＜0.05),a significant increase in infarct area(P＜0.05),significant reductions in vascular density and ratio(P＜0.05),significant increases in the mRNA and protein expression levels of RCAN1(P＜0.05),and significant increases in the levels of IL-1β and IL-6 in the peri-infarct area(P＜0.05).Compared with the model group,the electroacupuncture group had significant improvements in the above indicators(P＜0.05),and after RCAN1 knockdown,the effect of electroacupuncture was significantly weakened(P＜0.05).Conclusion Electroacupuncture improves motor functional recovery in mice with cere-bral ischemia by upregulating the expression of RCAN1 in the peri-infarct area,reducing infarct area,increasing vascular densi-ty,and inhibiting inflammatory response.关键词
梗死灶周围区/电针/RCAN1/血管密度/运动功能Key words
Peri-infarct area/Electroacupuncture/Regulator of calcineurin 1/Vascular density/Motor function分类
医药卫生引用本文复制引用
施慧敏,杨雨蝶,丁宇,许能贵,崔帅..电针通过调控RCAN1促进脑缺血小鼠血管新生与运动功能恢复[J].安徽中医药大学学报,2026,45(1):35-41,7.基金项目
安徽中医药大学人才支持计划项目(DT2400000222) （DT2400000222）
