草业科学2025，Vol.12Issue(12)：3098-3107,10.DOI:10.11829/j.issn.1001-0629.2024-0547
干旱和盐胁迫对巴天酸模种子萌发及幼苗生理特性的影响
Effects of drought and salt stress on seed germination and seedling physiological index of Rumex patientia
摘要
Abstract
The study examined the effects of drought and salt stress on seed germination characteristics and seedling physiological index of Rumex patientia.Drought and salt stress on the seeds and seedlings were simulated using PEG-6000 and NaCl solutions at varying concentrations,respectively;it also measured the germination percentage,germination potential,germination index,and the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT),and the contents of maldodicarboxaldehyde(MDA)and proline(Pro)in the seedlings.The results indicate that with the increase in the concentration of PEG-6000 simulating drought stress,the seed germination percentage,the germination potential,the germination index of R.patientia initially increased but declined later.Under low concentrations of PEG-6000 simulating drought stress,the germination parameters were not significant(P>0.05);under high concentrations of PEG-6000(≥20%)simulating drought stress,the germination parameters were significantly lower than those in the control group(P<0.05).Similarly,with the increase in the concentration of NaCl simulating salt stress,the germination percentage,germination potential,and germination index of R.patientia seeds gradually decreased;low concentrations of NaCl had no significant effect on the germination of seeds(P>0.05).Low NaCl concentrations affected the germination of R.patientia seeds,while high concentration NaCl(≥0.7%)inhibited the germination of R.patientia seeds(P<0.05).Under both stresses,SOD,POD,and CAT activities first increased and then decreased,while MDA content increased progressively under drought and fluctuated under salt stress.Pro content showed a complex rise-fall pattern with increasing salt concentration.Except for the CAT content in seedling leaves under 30%PEG-6000 simulated drought stress,which was significantly lower than that of the control group(P<0.05),whereas it was significantly higher under 10%PEG-6000 simulated drought stress than in the control group(P<0.05).All other physiological parameters were higher than those of the control group,but the differences were not statistically significant(P>0.05).Therefore,R.patientia seeds and seedlings exhibit moderate drought and salt tolerance,though germination and growth are markedly inhibited under high-stress conditions.关键词
巴天酸模/PEG-6000/NaCl/种子萌发/生理指标/渗透调节物质/抗氧化酶活性Key words
Rumex patientia/PEG-6000/NaCl/seed germination/physiological index/osmotic regulation substances/antioxidant enzyme activity引用本文复制引用
李小莉,苏旭,刘玉萍,余明君,杨倩,孙成林,杨萍,陈玉敏..干旱和盐胁迫对巴天酸模种子萌发及幼苗生理特性的影响[J].草业科学,2025,12(12):3098-3107,10.基金项目
青海省重大科技专项(2023-SF-A5) （2023-SF-A5）
2023年中央林业草原生态保护恢复资金野生动植物保护项目(QHSY-2023-016) （QHSY-2023-016）
青海省省财政林业改革发展资金林草新技术推广项目(QSCZ-2023-001) （QSCZ-2023-001）
