草业学报2026，Vol.35Issue(1)：13-24,12.DOI:10.11686/cyxb2025065
氮磷添加对一年生人工草地土壤微生物-胞外酶生态化学计量特征的影响
Effects of nitrogen and phosphorus supplementation on ecological stoichiometric characteristics of soil microbial-extracellular enzymes in annual artificial grassland
摘要
Abstract
The aim of this research was to explore the effects of fertilization with different amounts of nitrogen and phosphorus on the characteristics of soil microbes.To this end,we prepared artificial grasslands with annual oat(Avena sativa)and alfalfa(Medicago sativa)grown alone as monocultures or as a mixture(1∶1).The experiment consisted of a control,N0P0(CK)and eight treatments,namely N0P2.5,N0P5,N5P0,N5P2.5,N5P5,N10P0,N10P2.5,and N10P5.These treatments represented combinations of N fertilizer at three levels(elemental N at 0,75 and 150 kg·ha-1)and phosphate fertilizer at three levels(elemental P at 0,37.5 and 75 kg·ha-1).We determined the carbon,N,and P contents in soil microbial biomass,the activities of extracellular enzymes in soil,and their ecological stoichiometric ratios in the three types of artificial grassland under the different levels of N and P fertilization.It was found that:1)Addition of different levels of N and P significantly affected the nutrient metabolism characteristics of soil microorganisms.In the oat monoculture,the fertilization treatment with high N and low P(N10P2.5)had a significant inhibitory effect on the carbon and N contents of soil microbial biomass.In the alfalfa monoculture,combined application of low N and high P(N5P5)resulted in increased carbon and N contents in soil microbial biomass.In the oat-alfalfa mixture,the influence of fertilization treatments on soil microbial biomass was complex,but fertilization with high P(N0P5)had an inhibitory effect on soil microbial biomass.2)The activities of β-1,4-glucosidase,leucine aminopeptidase,and alkaline phosphatase in the oat monoculture were greatly affected by the N and P fertilization treatments,while those in the alfalfa monoculture and the mixed oat-alfalfa grassland were relatively stable across all the treatments.The soil N∶P enzyme activity ratios were lower than 1 in all three grassland types,indicating that the P limitation for soil microorganisms at the experimental site was greater than the N limitation.In summary,for grasslands in the northern part of Shanxi Province,the combined application of low N and high P(N at 75 kg·ha-1 and P at 75 kg·ha-1)is optimal for oat and alfalfa as monocultures.However,for mixed oat-alfalfa sowings,low N and low P(N at 75 kg·ha-1 and P at 37.5 kg·ha-1)should be prioritized.关键词
人工草地/氮磷添加/微生物生物量碳氮磷/土壤胞外酶/生态化学计量比Key words
artificial grassland/nitrogen and phosphorus addition/microbial biomass carbon,nitrogen and phosphorus/soil extracellular enzymes/ecological stoichiometric ratio引用本文复制引用
康佳惠,郑敏娜,龚瑞杰,韩志顺,陈燕妮,梁秀芝..氮磷添加对一年生人工草地土壤微生物-胞外酶生态化学计量特征的影响[J].草业学报,2026,35(1):13-24,12.基金项目
山西省基础研究计划项目(202303021222076),山西农业大学科技创新提升工程(CXGC2023076),山西农业大学生物育种工程项目(YZGC087),山西省牧草产业技术体系(2025)和大同市应用基础研究计划项目(2024062)资助. （202303021222076）
