北京中医药2025，Vol.44Issue(12)：1543-1548,6.DOI:10.16025/j.1674-1307.2025.12.007
海藻玉壶汤加减海藻、甘草对甲状腺肿大鼠的作用及机制研究
Effects and mechanisms of Haizao Yuhu Decoction with or without Sargassum and Glycyrrhizae Radix et Rhizoma on goiter in rats
摘要
Abstract
Objective To investigate the effects and mechanisms of Haizao Yuhu Decoction(HYD)with or without Sargassum and Glycyrrhizae Radix et Rhizoma(GR)on goiter in rats.Methods A total of 128 rats were randomly divided into eight groups(16 rats per group)according to body weight,including blank group,model group,Euthyrox group,HYD group,HYD without Sargassum group(HYD-H),HYD without GR group(HYD-G),HYD without Sargassum and GR group(HYD-HG),and Sargassum-GR group(HG).The blank group received deionized water by gavage.The other groups were given propylthiouracil(PTU,10 mg/kg)by gavage once daily for 14 days to induce goiter.After successful modeling,except for the blank group,all other groups continued to receive PTU every other day.The control and model groups were given deionized water,the Euthyrox group received Euthyrox suspension(20 μg/kg),and the HYD,HYD-H,HYD-G,HYD-HG,and HG groups received corresponding decoctions of HYD(concentrations:1.206,0.990,1.026,0.810,and 0.396 g/mL,respectively)by gavage once daily for 14 days.Serum levels of T3,FT3,T4,FT4,and TSH were measured by ELISA.Thyroid weight and organ-to-brain ratio were compared.Thyroid ultrastructure was observed using transmission electron microscopy,and thyroid cell apoptosis was evaluated by TUNEL assay.Results Compared with the model group,thyroid swelling was alleviated in the HYD group and all decomposed formula groups.No significant differences were observed in organ-to-brain ratios among groups.Electron microscopy showed apoptotic features in all herbal treatment groups.TUNEL assay demonstrated that the apoptosis rate in the HYD group was significantly higher than in the model group(P<0.05).Conclusion HYD with or without Sargassum and GR significantly alleviates PTU-induced goiter in rats,and the therapeutic effect of the full HYD formula is superior to its decomposed versions.The mechanism may involve the induction of thyroid cell apoptosis.关键词
十八反/甲状腺肿/海藻玉壶汤/细胞凋亡/海藻/甘草Key words
eighteen antagonisms/goiter/Haizao Yuhu Decoction/apoptosis/Sargassum/Glycyrrhizae Radix et Rhizoma引用本文复制引用
田颐,修琳琳,钟赣生,董肖,曹灿,刘晓庆,廖文勇,徐向楠,吴美晶,陈绍红,柳海艳..海藻玉壶汤加减海藻、甘草对甲状腺肿大鼠的作用及机制研究[J].北京中医药,2025,44(12):1543-1548,6.基金项目
国家自然科学基金项目(82274118) （82274118）
