川北医学院学报2026，Vol.41Issue(1)：7-11,5.DOI:10.3969/j.issn.1005-3697.2026.01.002
大鼠脊神经结扎后脊髓背角神经元及突触数量的变化
Numerical changes of neurons and synapses in the rat spinal dorsal horn after spinal nerve ligation
摘要
Abstract
Objective:To explore the change and its significance of the numbers of neurons and synapses in the rat spinal dorsal horn at 7 and 28 days after L5 spinal nerve ligation(SNL).Methods:29 male SD rats aged 3 months were randomly divided into two groups:L5 unilateral SNL group(16 animals)and sham-operated group(13 animals).The mechanical withdrawal thresholds(PWT)of bilateral hind paws were measured every 4 days,starting from 1 day before the operation until 28 days post-operation.7 and 28 days post-operation,L5 segment of the spinal cord was removed.Paraffin-embedded sections were prepared and stained with Nissl's method and synaptophysin immunohistochemistry to mark neurons and synapses(presynaptic axon terminals).The numbers of neurons and synapses(per unit length of the spinal cord)in the L5 spinal cord dorsal horn were estimated with the stereological optical disector.Results:The percentage reductions of PWT on the operation side were significantly lower in the SNL group than in the sham-operated group.Compared with the contralateral non-operated side,the number of synapses and the numerical ratio of synapses to neurons were unchanged on the operation side in the SNL group at 7 days,and significantly increased by 23.5%and 17.4%respectively at 28 days.Conclusion:SNL-induced changes in the synaptic plasticity associated with neuropathic pain(NP)require prolonged development.This delayed neural remodeling may constitute the structural underpinnings of chronic pain maintenance.关键词
神经病性疼痛/体视学/脊髓背角/神经元/突触Key words
Neuropathic pain/Stereology/Spinal dorsal horn/Neuron/Synapse分类
医药卫生引用本文复制引用
黄小丽,彭彬,郭洋,向宇,杨正伟..大鼠脊神经结扎后脊髓背角神经元及突触数量的变化[J].川北医学院学报,2026,41(1):7-11,5.基金项目
四川省南充市市校科技战略合作专项(20SXQT0304) （20SXQT0304）
