渔业研究2026，Vol.48Issue(1)：85-95,11.DOI:10.14012/j.jfr.2025044
基于高分辨液相色谱与质谱联用系统分析银鲳肌肉脂质组成
Analysis of lipid composition in Pampus argenteus muscle using high-resolution liquid chromatography-mass spectrometry
摘要
Abstract
[Objective]This study aims to elucidate the lipid composition of silver pomfret(Pampus argenteus)muscle,establishing a scientific foundation for its potential applications in food processing and functional food development.[Methods]Wild P.argenteus samples were freeze-dried and ground into powder using liquid ni-trogen.Total lipids were extracted from the muscle samples using methanol and chloroform.The lipid profile in muscle of P.argenteus was explored by high-resolution liquid chromatography-mass spectrometry equipped with a HESI-Ⅱ probe.[Results]A total of 447 lipids belonging to 17 major classes were identified in P.ar-genteus.These included 4 classes of sphingolipids:sphingosine(So),ceramide(Cer),monoglycosylceramide(CerG1),and sphingomyelin(SM);2 classes of neutral glycerolipids:diacylglycerol(DAG)and triacylglycerol(TAG);11 classes of phospholipids:lysophosphatidylcholine(LPC),phosphatidylcholine(PC),lysophos-phatidy-lethanolamine(LPE),phosphatidylethanolamine(PE),lysophosphatidylserine(LPS),phosphatidylser-ine(PS),lysophosphatidylglycerol(LPG),phosphatidylglycerol(PG),lysophosphatidylinositol(LPI),phos-phatidylinositol(PI),and phosphatidic acid(PA).Quantitative analysis demonstrated that neutral glycerolipids(54.93%)and phospholipids(43.82%)constituted the predominant lipid fractions.Triglycerides(TAG)repres-ented the most abundant lipid species(38.37%of total lipids),while phosphatidylcholine(25.32%)and phos-phatidylethanolamine(11.48%)emerged as the major phospholipid components.Fatty acid profiling identified 32 molecular species,comprising 11 saturated fatty acids(SFA),8 monounsaturated fatty acids(MUFA),and 13 polyunsaturated fatty acids(PUFA),with PUFA exhibiting marginally higher abundance than SFA and MUFA.The major fatty acids in P.argenteus muscle were palmitic acid(C16:0),oleic acid(C18:1),eicosapentaenoic acid(EPA),and docosahexaenoic acid(DHA).[Conclution]High-resolution liquid chromatography-mass spectrometry analysis demonstrated that P.argenteus muscle contains a diverse array of lipids,with notably high levels of DHA and EPA,supporting its classification as a nutritious seafood under the"Blue Granary"framework.关键词
银鲳/脂质/脂肪酸/高分辨液相色谱与质谱联用系统Key words
Pampus argenteus/lipids/fatty acids/high-resolution liquid chromatography-mass spectrometry分类
农业科技引用本文复制引用
牟桐,黄莉莉,朱竹君,李艳荣,徐继林..基于高分辨液相色谱与质谱联用系统分析银鲳肌肉脂质组成[J].渔业研究,2026,48(1):85-95,11.基金项目
国家现代农业产业技术体系建设专项资金项目(CARS-49) （CARS-49）
浙江省自然科学基金项目(LTGC24D060001) （LTGC24D060001）
宁波市科技攻关项目(2024Z276) （2024Z276）
