中国妇幼健康研究2026，Vol.37Issue(1)：51-56,6.DOI:10.3969/j.issn.1673-5293.2026.01.008
TLR4、NF-κB在溃疡性结肠炎儿童血清中的表达及与预后复发的关系
Expression of TLR4 and NF-κB in serum of children with ulcerative colitis and their relationships with recurrence in prognosis
摘要
Abstract
Objective To investigate expression of toll-like receptor 4(TLR4)and nuclear transcription factor-κB(NF-κB)in serum of children with ulcerative colitis(UC)and their relationships with recurrence in prognosis.Methods A total of 80 children with UC who were treated in Department of Pediatrics,The First Huzhou Municipal People's Hospital over a period from November 2021 to November 2023 were selected in UC group,and according to disease severity,these children were further divided into three subgroups:mild UC subgroup(n=30),moderate UC subgroup(n=28)and severe UC subgroup(n=22).Other 30 healthy children who received physical examination in the same period were selected as healthy control group.The expression levels of TLR4 mRNA and NF-κB mRNA in peripheral blood of the children were compared among the four groups.Spearman correlation analysis was used to analyze correlations of TLR4 and NF-κB in peripheral blood with severity of UC.In addition,according to whether the UC recurred at follow-up after 6 months,the children in the UC group were divided into recurrence group(n=21)and non-recurrence group(n=59).Multivariate Logistic analysis was used to analyze influencing factors of UC recurrence in prognosis.Receiver operating characteristic(ROC)curve was used to analyze predictive efficacy of TLR4 and NF-κB for UC recurrence in prognosis.Results The expression levels of TLR4 mRNA and NF-κB mRNA in peripheral blood in the severe UC subgroup>those in the moderate UC subgroup>those in the mild UC subgroup>those in the healthy control group(all P<0.05).Spearman correlation analysis showed that the severity of UC disease was positively correlated with expression levels of TLR4 mRNA and NF-κB mRNA in peripheral blood(r=0.533 and 0.617 respectively,both P<0.05).Multivariate regression analysis showed that severe disease severity,total colonic type of UC(E4)in lesion range,no maintenance treatment after discharge,and elevated levels of TLR4 mRNA and NF-κB mRNA in peripheral blood were all independent risk factors for UC recurrence in prognosis(OR=1.793-2.243,all P<0.05).ROC curve analysis showed that the areas under the curve(AUCs)of TLR4 mRNA and NF-κB mRNA in peripheral blood and the combination of the two indexes for predicting UC recurrence in prognosis were 0.783,0.789 and 0.863 respectively,suggesting that the combined detection of the two indexes had the best predictive efficacy(P<0.05).Conclusion The up-regulated expressions of TLR4 mRNA and NF-κB mRNA in peripheral blood are two independent risk factor for UC recurrence in prognosis,and the combined detection of TLR4 mRNA and NF-κB mRNA is more effective in predicting UC recurrence in prognosis in children.关键词
儿童/溃疡性结肠炎/Toll样受体4/核转录因子-κB/预后复发Key words
children/ulcerative colitis/toll-like receptor 4/nuclear transcription factor-κB/recurrence in prognosis分类
医药卫生引用本文复制引用
钟亚敏,朱焰,沈锋强..TLR4、NF-κB在溃疡性结肠炎儿童血清中的表达及与预后复发的关系[J].中国妇幼健康研究,2026,37(1):51-56,6.基金项目
湖州市科学技术计划项目(2023GYB39) （2023GYB39）
