广西科学院学报2025，Vol.41Issue(4)：420-429,10.DOI:10.13657/j.cnki.gxkxyxb.20260107.007
2-甲基丙烯醛对杂环芳香胺2-氨基-3,8-二甲基咪唑并[4,5-f]喹喔啉(MeIQx)形成的影响
Effect of 2-Methyl Acrolein on the Formation of Heterocyclic Aromatic Amine 2-Amino-3,8-Dimethylimidazo[4,5-f]Qui-noxaline(MeIQx)
摘要
Abstract
To investigate the effect of 2-methyl acrolein,a lipid oxidation product,on the formation of hetero-cyclic aromactic amines 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline(MeIQx),a chemical model con-taining glycine,creatinine and glucose as well as a roasted tilapia surimi system were used to systematically analyze the mechanism of 2-methyl acrolein by Ultra-High Performance Liquid Chromatography-Mass Spec-trometry(UHPLC-MS)and Solid Phase Micro-Extraction Gas Chromatography-Mass Spectrometry(SPME-GC-MS).The results showed that the effect of 2-methyl acrolein on the formation of MeIQx was concentra-tion-dependent.The addition of 0.02-0.20 mmol/L 2-methyl acrolein could significantly promote the forma-tion of MeIQx,and the promotion effect disappeared when the concentration was higher than 0.20 mmol/L.The concentration of 0.80 mmol/L significantly inhibited the formation of MeIQx.2-Methyl acrolein can re-duce the residues of glycine and creatinine in the chemical model,increase the residue of glucose,and promote the formation of volatile intermediates such as glyoxal(GO),methylglyoxal(MGO)and 2,5-dimeth-ylpyrazine.Further verification found that 2-methyl acrolein could react with glycine,creatinine and MeIQx,and leaded to its consumption.In summary,2-methyl acrolein comprehensively affects the formation of MeIQx by promoting the Strecker degradation of glycine,promoting the formation of volatile intermediates,and reacting with MeIQx precursors and products.In addition,mild to moderate oxidation(within 4 d)of ti-lapia surimi could promote the formation of MeIQx,while highly oxidized(within 10 d)of tilapia surimi was the opposite.This study clarifies the regulation rule and mechanism of 2-methyl acrolein on the formation of MeIQx,provides a scientific basis for lipid oxidation products as a marker of MeIQx formation process,and has important reference value for optimizing food processing technology and reducing the risk of food-borne heterocyclic aromatic amines.关键词
2-甲基丙烯醛/MeIQx/前体/中间体/罗非鱼Key words
2-methyl acrolein/MeIQx/precursor/intermediate/tilapia分类
轻工纺织引用本文复制引用
刘奇峰,李文红,杨皓予,吕敏,蒋光正,覃琦云,潘传燕,罗旭,魏威豪,庞基堃..2-甲基丙烯醛对杂环芳香胺2-氨基-3,8-二甲基咪唑并[4,5-f]喹喔啉(MeIQx)形成的影响[J].广西科学院学报,2025,41(4):420-429,10.基金项目
广西自然科学基金项目(2025GXNSFFA069006),广西重点研发计划项目(桂科AB25069032)和八桂青年拔尖人才项目资助. （2025GXNSFFA069006）
