环球中医药2026，Vol.19Issue(1)：27-35,9.DOI:10.3969/j.issn.1674-1749.2026.01.004
强力益气颗粒对H2O2损伤大鼠L6骨骼肌成肌细胞的作用及机制研究
Study on the effects and mechanisms of Qiangli Yiqi Granules on H2O2-injured L6 skeletal muscle myoblasts in rats
摘要
Abstract
Objective By observing the effect of Qiangli Yiqi Granule on myoblasts of L6 skeletal muscle in rats injured by H2O2,the possible mechanism of action of MED 13/Nurr1/GLUT-4,a member of nuclear receptor 4 family,in MG was discussed.Methods After the L6 skeletal muscle myoblasts were isolated and cultured,they were divided into six groups:the normal serum group,the model group,the blank serum group,and the high,medium and low dose groups of serum containing Qiangyiqi Granule.Except for the normal serum group,all the other groups used H2O2 to cause oxidative damage.The normal serum group and the model group were cultured in FBS complete medium respectively.The blank serum group was incubated with normal rat serum(excluding the influence of PBS on cells),while the high,the medium and the low dose groups of Qiangyiqi medicated serum were incubated with rat medicated serum in corresponding dose groups,and the cells were collected after 24 hours of culture.Cell proliferation and apoptosis cycle were detected.Real-time PCR,immunofluorescence and Western blot were used to detect the quantitative,localized and qualitative expressions of Qiangli Yiqi serum on L6 skeletal muscle myofibrils PGC-1α,MEF2,MED13,Nurr1 and GLUT-4 protein.Results H2O2 treatment for 2 hours of the proliferation of myoblasts was significantly inhibited in L6 rats(P＜0.05),and the apoptosis rate was significantly increased(P＜0.05).The medicated serum of Qiangli Yiqi Granule can improve the proliferation rate of myoblasts in L6 skeletal muscles of rats injured by H2O2 and reduce the transfer to green.MED13 gene was significantly up-regulated at transcription level and protein level(P＜0.05).Compared with the model group,the transcription level of Nurr1 gene was decreased(P＜0.05).For PGC-1α,MEF2 and GLUT-4,H2O2 treatment significantly reduced their transcription levels(P＜0.05),and pretreatment of drug-containing serum could reverse this extent(P＜0.05),and at the same time a certain dose dependence was showed.Conclusion The medicated serum of Qiangli Yiqi Granule has a protective effect on L6 skeletal muscle myoblasts injured by H2O2 by increasing the protein levels of MED13,GLUT-4,PGC-1α,MEF2,and decreasing the protein level of Nurr1.It is concluded that the medicated serum of Qiangli Yiqi Granule can improve the glucose metabolism of skeletal muscle,reduce the damage of skeletal muscle cells,and further improve the pathology of impaired function of MG skeletal muscle by interfering with MED13/Nurr1/GLUT4 signaling pathway.关键词
重症肌无力/大鼠L6骨骼肌成肌细胞/强力益气颗粒/骨骼肌糖代谢/中介复合体13/核受体4家族成员2/葡萄糖转运蛋白-4信号途径Key words
myasthenia gravis/rat L6 skeletal muscle myoblasts/Qiangli Yiqi Granules/skeletal muscle glucose metabolism/Mediator complex 13/nuclear receptor 4 family member 2/glucose transporter-4 signaling pathway分类
医药卫生引用本文复制引用
陈建,李慧凛,应汝炯,董云,沈洁,吴丽琳,盛昭园..强力益气颗粒对H2O2损伤大鼠L6骨骼肌成肌细胞的作用及机制研究[J].环球中医药,2026,19(1):27-35,9.基金项目
上海市卫生健康委员会"海派中医流派传承延伸计划"(ZY(2021-2023)-0209-07) （ZY(2021-2023）
"十四五"中医特色专科建设(肌病专科)(ZYTSZK2-5) （肌病专科）
虹口区第二轮"国医强优"三年行动计划(2022-2024年)(HKGYQYXM-2022-15) （2022-2024年）
上海市虹口区卫生健康委员会中医药科研课题(HKQGYQY-ZYY-2023-05,HKQGYQY-ZYY-2023-04) （HKQGYQY-ZYY-2023-05,HKQGYQY-ZYY-2023-04）
上海中医药大学第二十四期课程建设项目 （）
上海中医药大学2022年度应对奥密克戎感染新型冠状病毒肺炎应急攻关项目(2022YJ-44) （2022YJ-44）
上海中医药大学科技发展基金项目资助(23KFL087) （23KFL087）
