湖南农业大学学报（自然科学版）2025，Vol.51Issue(6)：63-68,6.DOI:10.13331/j.cnki.jhau.2025.06.007
辣椒和烟草瞬时转化中3种报告基因系统表型的比较分析
Comparison analysis of transient expression phenotypes for three reporter gene systems in pepper and tobacco
摘要
Abstract
Plant transformation vectors pBI121-antiPDS,pTRV2-PDS,and 1300flag-Ruby,each driven by the 35S promoter were constructed using antisense PDS(antiPDS),RUBY,and virus-induced gene silencing(VIGS)-mediated PDS silencing as reporter gene systems,respectively.Transient transformation of these vectors in tobacco and pepper was performed via Agrobacterium tumefaciens infiltration.The expression effects of the three reporter gene systems were evaluated based on the phenotypic outcomes resulted from transient expressions of antiPDS interference,VIGS,and RUBY.All three reporter genes systems were first validated in tobacco leaves and then introduced into pepper leaves.The results showed that each system produced discernible phenotypic changes in both species.Both VIGS and antiPDS effectively silenced PDS,leading to localized bleaching patches in infiltrated leaves.VIGS induced more extensive and systemic silencing,consistent with its viral propagation nature,whereas antiPDS caused only localized suppression without spreading.Transient expression of RUBY produced red pigmentation,which confined to the infiltration zones.Among the three reporter systems tested,VIGS-mediated silencing produced the most visually striking phenotype in both tobacco and pepper.However,the bleaching caused by PDS silencing impaired photosynthetic function,which consequently hindered the subsequent growth and regeneration of transformed tissues,thereby limiting its use to transient assays.In contrast,the RUBY system acts as a gain-of-function reporter without adverse effects on plant development,suggesting it is a more suitable visual marker for applications requiring stable genetic transformation in pepper.关键词
辣椒/烟草/报告基因/瞬时表达Key words
pepper/tobacco/reporter genes/transient expression分类
生物科学引用本文复制引用
刘芬,罗婕,滕慧,王育捷,赵燕,张学文..辣椒和烟草瞬时转化中3种报告基因系统表型的比较分析[J].湖南农业大学学报（自然科学版）,2025,51(6):63-68,6.基金项目
国家自然科学基金项目(U21A20230) （U21A20230）
