福建农业学报2025，Vol.40Issue(10)：987-996,10.DOI:10.19303/j.issn.1008-0384.2025.10.002
药用菊花花型性状与ISSR和SCoT标记关联分析
ISSR and SCoT Molecular Markers Related to Floral Phenotypes of Chrysanthemum morifolium for medicine
摘要
Abstract
[Objective]Molecular markers closely related to the floral phenotypes of Chrysanthemum morifolium for medicine were identified to facilitate breeding selection.[Methods]Based on 11 ISSR and 20 SCoT markers,64 accessions of medicinal chrysanthemum(C.morifolium)were scanned for multiple genomic loci.On selected 9 floral phenotype traits,genetic diversity and population structure were analyzed using OriginPro 2024 and Structure 2.3 software.Association of the traits with the ISSR/SCoT markers was examined with Tassel 5.0.[Results]The coefficients of variation on the 9 traits ranged from 30.67%to 82.72%.The markers generated 197 polymorphic loci with polymorphic information contents between 0.57 and 0.87,averaging 0.78.Significant or extremely significant correlation was observed between most of the traits.The 64 accessions were clustered into three primary classes by flower size,i.e.,large,small,and intermediate.And,at the optimal grouping at K=4(ΔKmax)for classifying population structure,they were divided into 4 subpopulations that covered 81.25%of all with a membership probability(Q)greater than 0.6.Out of the 31 markers,12 loci significantly correlated with 8 flower traits(P＜0.01)explaining 11.74%-29.27%of the phenotypic variations.And the association between ISSR37 and the length of ray floret was most significant.[Conclusion]The collected 64 C.morifolium germplasms for medicine were genetically rich in diversity on the floral phenotypes but relatively simple in relationship.The 12 trait-related ISSR/SCoT markers identified in this study could be of value for breeding selection.关键词
药用菊花/种质资源/花型性状/ISSR分子标记/SCoT分子标记Key words
Chrysanthemum morifolium/germplasm resources/floral phenotype traits/ISSR molecular markers/SCoT molecular markers分类
农业科技引用本文复制引用
许兰杰,余永亮,安素妨,董薇,吴晓慧,杨青,冯俊平,杨红旗,梁慧珍..药用菊花花型性状与ISSR和SCoT标记关联分析[J].福建农业学报,2025,40(10):987-996,10.基金项目
国家现代农业产业技术体系建设专项(CARS-21) （CARS-21）
河南省重点研发专项(241111310200、231111110800) （241111310200、231111110800）
河南省自主创新项目(2025ZC44) （2025ZC44）
河南省科技攻关项目(222102110466、222102110379) （222102110466、222102110379）
