福建农业学报2025，Vol.40Issue(10)：1006-1013,8.DOI:10.19303/j.issn.1008-0384.2025.10.004
金定鸭与龙岩山麻鸭的选择信号差异分析
Differences on Genetic Selection Signals of Jinding Ducks and Longyan Shanma Ducks
摘要
Abstract
[Objective]Selection signals of egg-laying Jinding duck and Longyan Shanma duck for effective identification of target genes and pathways for breeding were investigated.[Method]Thirty Jinding ducks and Longyan Shanma ducks each were randomly selected for whole-genome resequencing.Based on the sequences,GATK was used to obtain single nucleotide polymorphisms(SNPs)and conduct principal component analysis(PCA).Subsequently,the population genetic differentiation index,Fst,and the population nucleotide diversity ratio,π ratio,were used to comprehensively screen for selection signals.Candidate genes were subjected to GO and KEGG enrichment analysis for functional confirmations.[Result]A vast number of 307 247 849 SNPs were obtained by the resequencing.Significant genetic differentiations between Jinding duck and Longyan Shanma duck were indicated by PCA.The Fst and π ratio calculated by using a sliding window of 100 kb and a step size of 10 kb,respectively,were separated by the following thresholds into groups of top 5%Fst at Fst＞0.353,top 5%π ratio at π ratio≥1.49,and bottom 5%π ratio at 0＜π ratio≤0.41.As a result,200 annotated genes in the overlapping signal regions were identified to be the candidates for the selection.Subsequently,analyses revealed that 79 of these genes were significantly enriched in 204 GO functions and 5 KEGG pathways.And the functional genes related to the economic traits of interest were identified to include ELOVL7,HACD4,JAK2,ETV1,HOMER1,HTR1D,ACVR2A,PCSK5,SLC1A3,ANXA1,PTAFR,INHBB,and IL31RA.[Conclusion]Genetically,Jinding duck and Longyan Shanma duck were significantly different.This study identified selection signals of 13 genes related to lipid metabolism,growth and development,immunity,and reproduction that could significantly facilitate breeding fowls with desired traits.关键词
金定鸭/龙岩山麻鸭/全基因组重测序/选择信号/候选基因Key words
Jinding duck/Longyan Shanma duck/whole genome resequencing/selection signal/candidate genes分类
农业科技引用本文复制引用
朱志明,周世业,黄种彬,林炜,郑嫩珠,刘长涛,潘成福,李丽,辛清武,缪中纬,章琳俐,刘晓盼,黄靓..金定鸭与龙岩山麻鸭的选择信号差异分析[J].福建农业学报,2025,40(10):1006-1013,8.基金项目
国家重点研发计划项目(2024YFD1300902) （2024YFD1300902）
福建省科技重大专项(2024NZ029028) （2024NZ029028）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021008) （XTCXGC2021008）
福建省种业创新与产业化工程项目(zycxny2021014) （zycxny2021014）
