福建农业学报2025，Vol.40Issue(10)：1030-1039,10.DOI:10.19303/j.issn.1008-0384.2025.10.007
东北草莓DREB1B基因克隆及表达分析
Cloning and Expression of DREB1B in Fragaria mandschurica Staudt
摘要
Abstract
[Objective]Involvement of DREB1B in the cold resistance of Fragaria mandschurica Staudt was studied.[Methods]Full length cDNA of DREB1B in F.mandschurica was cloned by RT-PCR and analyzed bioinformatically.The sequence of FmDREB1B promoter was cloned with cis-acting elements predicted.GUS vector was constructed to be transiently transformed into tobacco leaves for staining and enzyme activity determination.Relative expression of FmDREB1B was analyzed using RT-qPCR.[Results]The cDNA of FmDREB1B(GenBank accession number:MN738503.1)was 690 bp in length encoding 229 amino acids that included a conserved AP2 DNA-binding domain.The amino acids encoded by FmDREB1B had the highest homology with FvDREB1B.The FmDREB1B promoter(GenBank accession number:MN933919.1)contained several cis-acting elements associated with hormones and adversity stress responses.The transformation of pFmDREB1B::GUS into tobacco leaves resulted in the transcriptional activity of the promoter.DREB1B expressed in various tissues of F.mandschurica that peaked to be 22.58 times of 0 h after 6 h at 4℃.A 6 h ABA(abscisic acid)treatment produced a peak expression 15.28 times the original.In contrast,in F.ananassa Duch,the highest expression of DREB1B was 5.41-folds of that at 0 h under the same treatment at 4℃for 6 h,while the ABA treatment resulted in merely 4.64-folds of 0 h.[Conclusion]The finding that DREB1B is involved in the low-temperature response,combined with its initial identification and functional characterization,lays a foundation for further research into its role in the cold tolerance mechanism of F.mandschurica.关键词
东北草莓/低温/DREB1B/基因表达Key words
Fragaria mandschurica Staudt/low temperature/DREB1B/gene expression分类
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孙营博,林小雨,施红云,雷天赐,张梦琪,陈清西,文志丰..东北草莓DREB1B基因克隆及表达分析[J].福建农业学报,2025,40(10):1030-1039,10.基金项目
国家自然科学基金项目(32072526) （32072526）
