福建农业学报2025，Vol.40Issue(10)：1048-1056,9.DOI:10.19303/j.issn.1008-0384.2025.10.009
康县核桃叶斑病病原鉴定及复配药剂筛选
Pathogen Identification and Compound Fungicide of Walnut Leaf Spot Disease at Kang County
摘要
Abstract
[Objective]Pathogen causing walnut leaf spots at Kang County and effective fungicide for the disease control were identified.[Method]Probable pathogen was isolated by tissue isolation to be identified by morphological examination,pathogenicity tests,and sequences of ITS,TUB2,and RPB2.Inhibition effects of fungicides on the pathogen were determined according to mycelium growth with EC50 calculated.[Result]Alternaria yaliinficiens was identified to be the pathogen that caused the walnut leaf spot disease at Kang County.The in vitro test showed 8 fungicides that significantly inhibited the mycelial growth of the pathogen.Among them,97%Prochloraz technical exhibited the greatest inhibition with an EC50 of 0.706 9 mg·L-1,which was followed by 98%Procymidone technical,96%Iprodione technical,and 80%Mancozeb technical with EC50 of 1.244 4 mg·L-1,1.332 2 mg·L-1,and 7.115 2 mg·L-1,respectively.By combining two fungicides,synergistic pathogen-inhibitory effects were found in 97%Prochloraz technical and 97%Difenoconazole technical at the mixing volume ratio of 5:1 with a coefficient(SR)of 1.82,and at 3:1 volume ratio with an SR of 1.67 or at 1:1 volume ratio with an SR of 2.51.And the combination of 97%Difenoconazole technical and 97%Tebuconazole technical in the volume ratios of 5:1,3:1,1:1,1:3,and 1:5 with the SRs of 5.18,4.53,5.45,7.31,and 7.67,respectively,while that of 97%Difenoconazole technical and 97%Tebuconazole technical in the volume ratio of 5:1 with an SR of 7.67,the synergistic effect was the best.[Conclusion]For the first time,the pathogen of walnut leaf spot disease in China was identified as A.yaliinficiens.The fungicides,97%Prochloraz technical,98%Procymidone technical,96%Iprodione technical,and 80%Mancozeb technical,were significantly inhibitory to the pathogen as shown by in vitro test.Moreover,the combination of 97%Prochloraz technical with 97%Difenoconazole technical or 97%Difenoconazole technical with 97%Tebuconazole technical displayed significant synergistic inhibition effect.关键词
核桃叶斑病/形态学观察/致病性测定/多基因序列/复配药剂/康县Key words
walnut leaf spot disease/morphological examination/pathogenicity test/multi-locus sequence analysis/compound fungicide/Kang County分类
农业科技引用本文复制引用
王琳莉..康县核桃叶斑病病原鉴定及复配药剂筛选[J].福建农业学报,2025,40(10):1048-1056,9.基金项目
康县农业综合改革基金(GSAU-JSFW-2023-128) （GSAU-JSFW-2023-128）
