福建农业学报2025，Vol.40Issue(11)：1101-1109,9.DOI:10.19303/j.issn.1008-0384.2025.11.001
玉米ZmPHYB2基因的克隆及其在不同光周期条件下的表达分析
The Cloning of ZmPHYB2 Gene in Maize and its Expression Analysis in Different photoperiods
摘要
Abstract
[Objective]Expressions of ZmPHYB2 in the maize inbred lines from temperate and tropical regions exposed to varied photoperiods were determined to understand the phytochromic mechanism of the plants.[Method]The different maize inbred lines,CML288 from a tropical region and Huangzaosi from a temperate zone,were placed under night-break(NB)after a short 9 h photoperiod day(SD)for an extended period.Flowering of the plants were monitored.ZmPHYB2 were cloned using the homologous cloning method for a bioinformatic analysis,and expressions of the genes in 48 h under the treatment determined by qRT-PCR.[Result]NB significantly delayed flowering of the tropical CML288,but accelerated that of Huangzaosi.There were 19 single-nucleotide polymorphisms in the coding sequences(CDS)of ZmPHYB2 of the two lines,and 7 differentiated amino acids including 5 localized in two conserved domains.The changed photo-exposure significantly disrupted the ZmPHYB2 expression in the CML288 leaves and lowered the transcript level in the leaves of Huangzaosi.[Conclusion]It appeared that the 5 different amino acids in two conserved domains of ZmPHYB2,the perturbed ZmPHYB2 expression in CML288 leaves,and the downregulated ZmPHYB2 expression in Huangzaosi leaves introduced by NB contributed to the delayed flowering in the tropical CML288 in comparison to the temperate counterparts.关键词
玉米/暗间断/ZmPHYB2/光周期敏感/表达分析Key words
maize/night break/ZmPHYB2/photoperiod-sensitive/expression analysis分类
农业科技引用本文复制引用
韦小敏,库丽霞,苏慧慧..玉米ZmPHYB2基因的克隆及其在不同光周期条件下的表达分析[J].福建农业学报,2025,40(11):1101-1109,9.基金项目
国家自然科学基金项目(32201848) （32201848）
